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BMC Oral Health
Published in: BMC Oral Health 1/2021

Open Access 01-12-2021 | Digital Volume Tomography | Research

Genotoxicity and cytotoxicity of cone beam computed tomography in children

Authors: Doua H. Altoukhi, Sumer Alaki, Eman El Ashiry, Osama Nassif, Dania Sabbahi

Published in: BMC Oral Health | Issue 1/2021

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Abstract

Background

Dental radiographs are essential tools for diagnosis. However, there are significant concerns about the dangerous effect of radiation especially on children. The aim of this study was to evaluate genotoxicity and cytotoxicity in the exfoliated cells of buccal mucosa of children subjected to Cone Beam Computed Tomography (CBCT).

Methods

The study included 18 healthy children aged (9–12 years) who were exposed to CBCT. All CBCT scans were performed with the i-CAT CBCT. Exfoliated buccal cells were scraped from the left and right cheek immediately before the exposure, after 10 ± 2 days, and after 1 month. Cells were stained using Feulgen/fast green stain and examined under light microscopy. Genotoxicity (Micronuclei) and cytotoxicity (condensed chromatin, karyorrhexis, pyknosis, and karyolysis) were scored. Statistical analysis was performed using the McNemar test, Wilcoxon Signed-Rank test, and Mann-Whitney U test at a significance level of p < 0.05.

Results

There were statistically significant differences in the mean percentages of micronuclei, condensed chromatin, karyorrhexis, pyknosis, and karyolysis before and 10 ± 2 days after the CBCT scan (p < 0.05). There were no statistically significant differences in the frequency of micronuclei, condensed chromatin, karyorrhexis, or pyknosis before and 1 month after the exposure (p > 0.05) except for karyolysis (p < 0.05).

Conclusions

CBCT may induce genotoxicity and cytotoxicity in buccal mucosa cells of children. Therefore, CBCT should not be prescribed unless necessary as it cannot be considered a risk-free procedure.
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Metadata
Title
Genotoxicity and cytotoxicity of cone beam computed tomography in children
Authors
Doua H. Altoukhi
Sumer Alaki
Eman El Ashiry
Osama Nassif
Dania Sabbahi
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2021
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-021-01792-w

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