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Archives of Virology
Published in: Archives of Virology 2/2012

01-02-2012 | Brief Report

Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis

Authors: M. R. Islam, S. Rahman, M. Noor, E. H. Chowdhury, H. Müller

Published in: Archives of Virology | Issue 2/2012

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Abstract

Molecular characterization of IBDV usually relies on the analysis of segment A of the bi-segmented, double-stranded RNA genome. Although segments B of classical and very virulent IBDVs differ significantly, re-assortment of genome segments does occur, and molecular epidemiological studies demand the analysis of both segments. An RT-PCR and restriction enzyme analysis for molecular discrimination between genome segment B of classical and very virulent IBDVs is described. Tested on eight IBDV strains/isolates, the protocol successfully identified very virulent and classical IBDVs as well as a segment reassortant. This approach is a valuable tool for molecular epidemiological studies on IBDV.
Literature
1.
Leong JC, Brown D, Dobos P, Kibenge F, Ludert JE, Müller H, Mundt E, Nicholoson B (2000) Birnaviridae. In: Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds) Virus taxonomy: classification and nomenclature of viruses. Academic Press, New York, pp 481–490
2.
Müller H, Scholtissek C, Becht H (1979) The genome of infectious bursal disease virus consists of two segment of double-stranded RNA. J Virol 31:584–589PubMed
3.
von Einem UI, Gorbalenya AE, Schirrmeier H, Behrens SE, Letzel T, Mundt E (2004) VP1 of infectious bursal disease virus is an RNA-dependent RNA polymerase. J Gen Virol 85:2221–2229CrossRef
4.
Müller H, Islam MR, Raue R (2003) Research on infectious bursal disease–the past, the present and the future. Vet Microbiol 97:153–165PubMedCrossRef
5.
McFerran JB, McNulty MS, McKillop ER, Conner TJ, McCracken RM, Collins DS, Allan GM (1980) Isolation and serological studies with infectious bursal disease viruses from fowl, turkey and duck: demonstration of a second serotype. Avian Pathol 9:395–404PubMedCrossRef
6.
Islam MR, Zierenberg K, Müller H (2001) The genome segment B encoding the RNA dependent RNA polymerase protein VP1 of very virulent infectious bursal disease virus (IBDV) is phylogenetically distinct from that of all other IBDV strains. Arch Virol 146:2481–2492PubMedCrossRef
7.
Zierenberg K, Raue R, Nieper H, Islam MR, Eterradossi N, Toquin D, Müller H (2004) Generation of serotype 1/serotype 2 reassortant viruses of the infectious bursal disease virus and their investigation in vitro and in vivo. Virus Res 105:23–34PubMedCrossRef
8.
Liu M, Vakharia VN (2004) VP1 protein of infectious bursal disease virus modulates the virulence in vivo. Virology 330:62–73PubMedCrossRef
9.
Li L, Huang YW, Wang LS, Wan WJ, Yu L (2005) Synthesis of reassortant infectious bursal disease virus in chickens injected directly with infectious clones from different virus strains. Acta Biochem Biophys Sin (Shanghai) 37:192–198
10.
Boot HJ, Hoekman AJ, Gielkens AL (2005) The enhanced virulence of very virulent infectious bursal disease virus is partly determined by its B-segment. Arch Virol 150:137–144PubMedCrossRef
11.
Sun JH, Lu P, Yan YX, Hua XG, Jiang J, Zhao Y (2003) Sequence and analysis of genomic segment A and B of very virulent infectious bursal disease virus isolated from China. J Vet Med B 50:148–154CrossRef
12.
Le Nouën C, Rivallan G, Toquin D, Darlu P, Morin Y, Beven V, de Boisseson C, Cazaban C, Comte S, Gardin Y, Eterradossi N (2006) Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate. J Gen Virol 87:209–216PubMedCrossRef
13.
Gao HL, Wang XM, Gao YL, Fu CY (2007) Direct evidence of reassortment and mutant spectrum analysis of a very virulent infectious bursal disease virus. Avian Dis 51:893–899PubMedCrossRef
14.
Brandt M, Yao K, Liu M, Heckert RA, Vakharia VN (2001) Molecular determinants of virulence, cell tropism, and pathogenic phenotype of infectious bursal disease virus. J Virol 75:11974–11982PubMedCrossRef
15.
Jackwood DJ, Jackwood RJ (1994) Infectious bursal disease virus: molecular differentiation of antigenic subtypes among serotype 1 viruses. Avian Dis 38:531–537PubMedCrossRef
16.
Zierenberg K, Raue R, Müller H (2001) Rapid identification of “very virulent” strains of infectious bursal disease virus by reverse transcription polymerase chain reaction combined with restriction enzyme analysis. Avian Pathol 30:55–62PubMedCrossRef
17.
Badhy SC, Das PM, Islam MR (2004) Detection and characterization of infectious bursal disease virus of chickens by reverse transcription - polymerase chain reaction and restriction enzyme analysis. Bangladesh Vet J 38:43–52
18.
Islam MR, Zierenberg K, Eterradossi N, Toquin D, Rivallan G, Müller H (2001) Molecular and antigenic characterization of Bangladeshi isolates of infectious bursal disease virus demonstrates their similarities with recent European, Asian and African very virulent strains. J Vet Med B 48:211–221CrossRef
19.
van den Berg TP, Gonze M, Meulemans G (1991) Acute infectious bursal disease in poultry: isolation and characterization of a highly virulent strain. Avian Pathol 20:133–143PubMedCrossRef
20.
Zhang MF, Qiao S, Zhu Y (2001) Molecular epidemiology of infectious bursal disease virus in China. In: Proceedings of the 2nd international symposium on infectious bursal disease and chicken infectious anaemia. Giessen, Germany. pp 194–203
21.
Bygrave AC, Faragher JT (1970) Mortality associated with Gumboro disease. Vet Rec 86:758–759
22.
Nick H, Cursiefen D, Becht H (1976) Structural and growth characteristics of infectious bursal disease virus. J Virol 18:227–234PubMed
23.
Wei Y, Li J, Zheng J, Xu H, Li L, Yu L (2006) Genetic reassortment of infectious bursal disease virus in nature. Biochem Biophys Res Commun 17:277–287CrossRef
24.
Xia RX, Wang HY, Huang GM, Zhang MF (2008) Sequence and phylogenetic analysis of a Chinese very virulent infectious bursal disease virus. Arch Virol 153:1725–1729PubMedCrossRef
25.
Yu F, Qi X, Yuwen Y, Wang Y, Gao H, Gao Y, Qin L, Wang X (2010) Molecular characteristics of segment B of seven very virulent infectious bursal disease viruses isolated in China. Virus Genes 41:246–249PubMedCrossRef
Metadata
Title
Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis
Authors
M. R. Islam
S. Rahman
M. Noor
E. H. Chowdhury
H. Müller
Publication date
01-02-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-011-1159-9

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