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Critical Care

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Open Access 01-04-2010 | Research

Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation

Authors: Oh Yoen Kim, Antoine Monsel, Michèle Bertrand, Pierre Coriat, Jean-Marc Cavaillon, Minou Adib-Conquy

Published in: Critical Care | Issue 2/2010

Abstract

Introduction

Decreased expression of human leukocyte antigen class II (HLA-DR) on monocytes is a hallmark of altered immune status in patients with a systemic inflammatory response syndrome (SIRS). So far, the analyses were mainly performed without taking into account monocytes subpopulations.

Methods

We studied this modification on CD14^HIGH and CD14^LOW monocytes of 20 SIRS patients undergoing abdominal aortic surgery (AAS), 20 patients undergoing carotid artery surgery (CAS), and 9 healthy controls, and we investigated mediators and intracellular molecules that may be involved in this process.

Results

HLA-DR on CD14^HIGH monocytes started to decrease during surgery, after blood reperfusion, and was further reduced post-surgery. In contrast, HLA-DR expression on CD14^LOW cells only decreased after surgery, and to a lesser extent than on CD14^HIGH monocytes. Negative correlations were found between the reduction of HLA-DR expression and the change in cortisol levels for both subpopulations, whereas a negative correlation between interleukin-10 (IL-10) levels and HLA-DR modulation was only observed for CD14^HIGH cells. In accordance with these ex vivo results, HLA-DR on CD14^HIGH and CD14^LOW monocytes of healthy donors was reduced following incubation with hydrocortisone, whereas IL-10 only acted on CD14^HIGH subpopulation. Furthermore, flow cytometry revealed that the expression of IL-10 receptor was higher on CD14^HIGH versus CD14^LOW monocytes. In addition, hydrocortisone, and to a lesser extent IL-10, reversed the up-regulation of HLA-DR induced by bacterial products. Finally, membrane-associated RING-CH-1 protein (MARCH1) mRNA, a negative regulator of MHC class II, was up-regulated in monocytes of AAS patients on Day 1 post-surgery, and in those of healthy subjects exposed to hydrocortisone.

Conclusions

This study reveals that HLA-DR expression is modulated differently on CD14^HIGH (classical) versus CD14^LOW (inflammatory) monocytes after systemic inflammation.

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Title: Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation
Authors: Oh Yoen Kim
Antoine Monsel
Michèle Bertrand
Pierre Coriat
Jean-Marc Cavaillon
Minou Adib-Conquy
Publication date: 01-04-2010
Publisher: BioMed Central
Published in: Critical Care / Issue 2/2010
Electronic ISSN: 1364-8535
DOI: https://doi.org/10.1186/cc8959

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation

Abstract

Introduction

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusions

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