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Pediatric Surgery International

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01-12-2019 | Diaphragmatic Hernia | Original Article

An intra-amniotic injection of mesenchymal stem cells promotes lung maturity in a rat congenital diaphragmatic hernia model

Authors: Shohei Takayama, Kohei Sakai, Shigehisa Fumino, Taizo Furukawa, Tsunao Kishida, Osam Mazda, Tatsuro Tajiri

Published in: Pediatric Surgery International | Issue 12/2019

Abstract

Purpose

We aimed to evaluate the effect of human mesenchymal stem cells (hMSCs) on congenital diaphragmatic hernia (CDH) by intra-amniotic injection in a rat CDH model.

Methods

Nitrofen (100 mg) was administered to pregnant rats at E9.5. hMSCs (1.0 × 10⁶) or PBS was injected into each amniotic cavity at E18, and fetuses were harvested at E21. The fetal lungs were classified into normal, CDH, and CDH-hMSCs groups. To determine the lung maturity, we assessed the alveolar histological structure by H&E and Weigert staining and the alveolar arteries by Elastica Van Gieson (EVG) staining. TTF-1, a marker of type II alveolar epithelial cells, was also evaluated by immunohistochemical staining and real-time reverse transcription polymerase chain reaction.

Results

The survival rate after intra-amniotic injection was 72.1%. The CDH-hMSCs group had significantly more alveoli and secondary septa than the CDH group (p < 0.05). The CDH-hMSCs group had larger air spaces and thinner alveolar walls than the CDH group (p < 0.05). The medial and adventitial thickness of the pulmonary artery in the CDH-hMSCs group were significantly better (p < 0.001), and there were significantly fewer TTF-1-positive cells than in the CDH group (p < 0.001).

Conclusion

These results suggest that intra-amniotic injection of hMSCs has therapeutic potential for CDH.

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Title: An intra-amniotic injection of mesenchymal stem cells promotes lung maturity in a rat congenital diaphragmatic hernia model
Authors: Shohei Takayama
Kohei Sakai
Shigehisa Fumino
Taizo Furukawa
Tsunao Kishida
Osam Mazda
Tatsuro Tajiri
Publication date: 01-12-2019
Publisher: Springer Berlin Heidelberg
Keywords: Diaphragmatic Hernia
Diaphragmatic Hernia
Published in: Pediatric Surgery International / Issue 12/2019
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-019-04561-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

An intra-amniotic injection of mesenchymal stem cells promotes lung maturity in a rat congenital diaphragmatic hernia model

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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