Top

European Journal of Clinical Microbiology & Infectious Diseases

Published in:

Open Access 01-06-2018 | Original Article

Diagnostic accuracy of the InBiOS AMD rapid diagnostic test for the detection of Burkholderia pseudomallei antigen in grown blood culture broth

Authors: Marjan Peeters, Panha Chung, Hua Lin, Kristien Mortelmans, Chhundy Phe, Chentha San, Laura Maria Francisca Kuijpers, Syna Teav, Thong Phe, Jan Jacobs

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 6/2018

Abstract

To assess the diagnostic and operational performance of the InBiOS AMD rapid diagnostic test (RDT) (Seattle, USA) for the detection of B. pseudomallei in grown blood culture broth. The InBiOS RDT is a lateral flow immunoassay in a strip format detecting B. pseudomallei capsular polysaccharide in culture fluids, marketed for research only. Broth of blood culture bottles (BacT/Alert, bioMérieux, Marcy L’Etoile, France) sampled in adult patients at the Sihanouk Hospital Center of HOPE, Phnom Penh, Cambodia, during 2010–2017 and stored at − 80 °C was tested. They included samples grown with B. pseudomallei (n = 114), samples with no growth (n = 12), and samples with growth of other pathogens (n = 139, among which Burkholderia cepacia (n = 5)). Diagnostic sensitivity and specificity were 96.5% [95% confidence interval (CI): 91.3–98.6%] and 100% [CI: 97.5–100%] respectively. Background clearance and line intensities were good and very good. The RDT’s test strip, not housed in a cassette, caused difficulties in manipulation and biosafety. The centrifugation step prescribed by the procedure challenged biosafety, but processing of 19 B. pseudomallei samples without centrifugation showed similar results for line intensity and background clearance, compared to centrifugation. The InBiOS RDT showed excellent accuracy for detection of B. pseudomallei in grown blood culture broth. Provided operational adaptations such as cassette housing, it has the potential to reduce time to diagnosis of melioidosis.

Available only for authorised users

Wiersinga WJ, Currie BJ, Peacock SJ (2012) Melioidosis. N Engl J Med 367(11):1035–1044. https://doi.org/10.1056/NEJMra1204699 CrossRefPubMed

Cheng AC, Currie BJ (2005) Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18(2):383–416. https://doi.org/10.1128/cmr.18.2.383-416.2005 CrossRefPubMedPubMedCentral

Chewapreecha C, Holden MT, Vehkala M, Valimaki N, Yang Z, Harris SR, Mather AE, Tuanyok A, De Smet B, Le Hello S, Bizet C, Mayo M, Wuthiekanun V, Limmathurotsakul D, Phetsouvanh R, Spratt BG, Corander J, Keim P, Dougan G, Dance DA, Currie BJ, Parkhill J, Peacock SJ (2017) Global and regional dissemination and evolution of Burkholderia pseudomallei. Nat Microbiol 2:16263. https://doi.org/10.1038/nmicrobiol.2016.263 CrossRefPubMedPubMedCentral

Limmathurotsakul D, Golding N, Dance DAB, Messina JP, Pigott DM, Moyes CL, Rolim DB, Bertherat E, Day NPJ, Peacock SJ, Hay SI (2016) Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis. Nat Microbiol 1(1):15008. https://doi.org/10.1038/nmicrobiol.2015.8 CrossRefPubMedCentral

Overtoom R, Khieu V, Hem S, Cavailler P, Te V, Chan S, Lau P, Guillard B, Vong S (2008) A first report of pulmonary melioidosis in Cambodia. Trans R Soc Trop Med Hyg 102(Suppl 1):S21–S25. https://doi.org/10.1016/s0035-9203(08)70007-5 CrossRefPubMed

Vlieghe ER, Phe T, De Smet B, Chhun Veng H, Kham C, Lim K, Koole O, Lynen L, Peetermans WE, Jacobs JA (2013) Bloodstream infection among adults in Phnom Penh, Cambodia: key pathogens and resistance patterns. PLoS One 8(3):e59775. https://doi.org/10.1371/journal.pone.0059775 CrossRefPubMedPubMedCentral

Currie BJ (2015) Melioidosis: evolving concepts in epidemiology, pathogenesis, and treatment. Semin Respir Crit Care Med 36(1):111–125. https://doi.org/10.1055/s-0034-1398389 CrossRefPubMed

Currie BJ, Ward L, Cheng AC (2010) The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study. PLoS Negl Trop Dis 4(11):e900. https://doi.org/10.1371/journal.pntd.0000900 CrossRefPubMedPubMedCentral

Vlieghe E, Kruy L, De Smet B, Kham C, Veng CH, Phe T, Koole O, Thai S, Lynen L, Jacobs J (2011) Melioidosis, Phnom Penh, Cambodia. Emerg Infect Dis 17(7):1289–1292. https://doi.org/10.3201/eid1707.101069 CrossRefPubMedPubMedCentral

10.

Mukhopadhyay C, Chawla K, Krishna S, Nagalakshmi N, Rao SP, Bairy I (2008) Emergence of Burkholderia pseudomallei and pandrug-resistant non-fermenters from southern Karnataka, India. Trans R Soc Trop Med Hyg 102(Suppl 1):S12–S17. https://doi.org/10.1016/s0035-9203(08)70005-1 CrossRefPubMed

11.

Limmathurotsakul D, Wongratanacheewin S, Teerawattanasook N, Wongsuvan G, Chaisuksant S, Chetchotisakd P, Chaowagul W, Day NP, Peacock SJ (2010) Increasing incidence of human melioidosis in Northeast Thailand. Am J Trop Med Hyg 82(6):1113–1117. https://doi.org/10.4269/ajtmh.2010.10-0038 CrossRefPubMedPubMedCentral

12.

Kingsley PV, Arunkumar G, Tipre M, Leader M, Sathiakumar N (2016) Pitfalls and optimal approaches to diagnose melioidosis. Asian Pac J Trop Med 9(6):515–524. https://doi.org/10.1016/j.apjtm.2016.04.003 CrossRefPubMed

13.

Houghton RL, Reed DE, Hubbard MA, Dillon MJ, Chen H, Currie BJ, Mayo M, Sarovich DS, Theobald V, Limmathurotsakul D, Wongsuvan G, Chantratita N, Peacock SJ, Hoffmaster AR, Duval B, Brett PJ, Burtnick MN, Aucoin DP (2014) Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis. PLoS Negl Trop Dis 8(3):e2727. https://doi.org/10.1371/journal.pntd.0002727 CrossRefPubMedPubMedCentral

14.

Peeters M (2017) Diagnostic performance of the InBiOS AMD rapid diagnostic test for detection of Burkholderia pseudomallei in blood culture broth and urine: results of a retrospective pilot study paper presented at the European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Vienna, Austria

15.

Perumal Samy R, Stiles BG, Sethi G, Lim LHK (2017) Melioidosis: clinical impact and public health threat in the tropics. PLoS Negl Trop Dis 11(5):e0004738. https://doi.org/10.1371/journal.pntd.0004738 CrossRefPubMedPubMedCentral

16.

Bossuyt PM, Reitsma JB, Bruns DE, Gatsonis CA, Glasziou PP, Irwig L, Lijmer JG, Moher D, Rennie D, de Vet HC, Kressel HY, Rifai N, Golub RM, Altman DG, Hooft L, Korevaar DA, Cohen JF (2015) STARD 2015: an updated list of essential items for reporting diagnostic accuracy studies. Clin Chem 61(12):1446–1452. https://doi.org/10.1373/clinchem.2015.246280 CrossRefPubMed

17.

Hodgson K, Engler C, Govan B, Ketheesan N, Norton R (2009) Comparison of routine bench and molecular diagnostic methods in identification of Burkholderia pseudomallei. J Clin Microbiol 47(5):1578–1580. https://doi.org/10.1128/jcm.02507-08 CrossRefPubMedPubMedCentral

18.

Samosornsuk N, Lulitanond A, Saenla N, Anuntagool N, Wongratanacheewin S, Sirisinha S (1999) Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis. Am J Trop Med Hyg 61(5):735–737CrossRefPubMed

19.

Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S (2000) Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49(12):1075–1078. https://doi.org/10.1099/0022-1317-49-12-1075 CrossRefPubMed

20.

InBiOS II (2013 ) Instructions for use : active melioidosis detect rapid test. Seattle, WA, USA

21.

Banoo S, Bell D, Bossuyt P, Herring A, Mabey D, Poole F, Smith PG, Sriram N, Wongsrichanalai C, Linke R, O’Brien R, Perkins M, Cunningham J, Matsoso P, Nathanson CM, Olliaro P, Peeling RW, Ramsay A (2010) Evaluation of diagnostic tests for infectious diseases: general principles. Nat Rev Microbiol 8(12 Suppl):S17–S29PubMed

22.

Maltha J, Gillet P, Cnops L, Bottieau E, Van Esbroeck M, Bruggeman C, Jacobs J (2011) Evaluation of the rapid diagnostic test SDFK40 (Pf-pLDH/pan-pLDH) for the diagnosis of malaria in a non-endemic setting. Malar J 10:7. https://doi.org/10.1186/1475-2875-10-7 CrossRefPubMedPubMedCentral

23.

Gillet P, Mori M, Van Esbroeck M, Van den Ende J, Jacobs J (2009) Assessment of the prozone effect in malaria rapid diagnostic tests. Malar J 8:271. https://doi.org/10.1186/1475-2875-8-271 CrossRefPubMedPubMedCentral

24.

Jacobs J, Barbe B, Gillet P, Aidoo M, Serra-Casas E, Van Erps J, Daviaud J, Incardona S, Cunningham J, Visser T (2014) Harmonization of malaria rapid diagnostic tests: best practices in labelling including instructions for use. Malar J 13:505. https://doi.org/10.1186/1475-2875-13-505 CrossRefPubMedPubMedCentral

25.

Nuti DE, Crump RB, Dwi Handayani F, Chantratita N, Peacock SJ, Bowen R, Felgner PL, Davies DH, Wu T, Lyons CR, Brett PJ, Burtnick MN, Kozel TR, AuCoin DP (2011) Identification of circulating bacterial antigens by in vivo microbial antigen discovery. MBio 2(4). https://doi.org/10.1128/mBio.00136-11

26.

Srinivasan A, Kraus CN, DeShazer D, Becker PM, Dick JD, Spacek L, Bartlett JG, Byrne WR, Thomas DL (2001) Glanders in a military research microbiologist. N Engl J Med 345(4):256–258. https://doi.org/10.1056/nejm200107263450404 CrossRefPubMed

27.

Whitlock GC, Estes DM, Torres AG (2007) Glanders: off to the races with Burkholderia mallei. FEMS Microbiol Lett 277(2):115–122. https://doi.org/10.1111/j.1574-6968.2007.00949.x CrossRefPubMed

28.

Van Zandt KE, Greer MT, Gelhaus HC (2013) Glanders: an overview of infection in humans. Orphanet J Rare Dis 8:131. https://doi.org/10.1186/1750-1172-8-131 CrossRefPubMedPubMedCentral

29.

Prevention CfDCa select agents and toxins list. Division of select agents and toxins. Accessed 22 Jan 2018

30.

De Smet B, Veng C, Kruy L, Kham C, van Griensven J, Peeters C, Ieng S, Phe T, Vlieghe E, Vandamme P, Jacobs J (2013) Outbreak of Burkholderia cepacia bloodstream infections traced to the use of Ringer lactate solution as multiple-dose vial for catheter flushing, Phnom Penh, Cambodia. Clin Microbiol Infect 19(9):832–837. https://doi.org/10.1111/1469-0691.12047 CrossRefPubMed

31.

Opota O, Jaton K, Greub G (2015) Microbial diagnosis of bloodstream infection: towards molecular diagnosis directly from blood. Clin Microbiol Infect 21(4):323–331. https://doi.org/10.1016/j.cmi.2015.02.005 CrossRefPubMed

32.

Gillet P, Scheirlinck A, Stokx J, De Weggheleire A, Chauque HS, Canhanga OD, Tadeu BT, Mosse CD, Tiago A, Mabunda S, Bruggeman C, Bottieau E, Jacobs J (2011) Prozone in malaria rapid diagnostics tests: how many cases are missed? Malar J 10:166. https://doi.org/10.1186/1475-2875-10-166 CrossRefPubMedPubMedCentral

33.

Robertson G, Sorenson A, Govan B, Ketheesan N, Houghton R, Chen H, AuCoin D, Dillon M, Norton R (2015) Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay. J Med Microbiol 64(8):845–848. https://doi.org/10.1099/jmm.0.000098 CrossRefPubMed

34.

Wongsuvan G, Limmathurotsakul D, Wannapasni S, Chierakul W, Teerawattanasook N, Wuthiekanun V (2009) Lack of correlation of Burkholderia pseudomallei quantities in blood, urine, sputum and pus. Southeast Asian J Trop Med Public Health 40(4):781–784PubMed

35.

Mohanty S, Pradhan G, Panigrahi MK, Mohapatra PR, Mishra B (2016) A case of systemic melioidosis: unravelling the etiology of chronic unexplained fever with multiple presentations. Pneumonol Alergol Pol 84(2):121–125. https://doi.org/10.5603/PiAP.2016.0012 CrossRefPubMed

36.

Frickmann H, Neubauer H, Haase G, Peltroche-Llacsahuanga H, Pérez-Bouza A, Racz P, Loderstaedt U, Hagen Ralf M (2013) Fatal urosepsis due to delayed diagnosis of genitourinary melioidosis1. Laboratoriumsmedizin 37. https://doi.org/10.1515/labmed-2013-0049

37.

Lau SK, Sridhar S, Ho CC, Chow WN, Lee KC, Lam CW, Yuen KY, Woo PC (2015) Laboratory diagnosis of melioidosis: past, present and future. Exp Biol Med (Maywood) 240(6):742–751. https://doi.org/10.1177/1535370215583801 CrossRef

38.

Wuthiekanun V, Chierakul W, Langa S, Chaowagul W, Panpitpat C, Saipan P, Thoujaikong T, Day NP, Peacock SJ (2006) Development of antibodies to Burkholderia pseudomallei during childhood in melioidosis-endemic Northeast Thailand. Am J Trop Med Hyg 74(6):1074–1075PubMed

39.

Pumpuang A, Dunachie SJ, Phokrai P, Jenjaroen K, Sintiprungrat K, Boonsilp S, Brett PJ, Burtnick MN, Chantratita N (2017) Comparison of O-polysaccharide and hemolysin co-regulated protein as target antigens for serodiagnosis of melioidosis. PLoS Negl Trop Dis 11(3):e0005499. https://doi.org/10.1371/journal.pntd.0005499 CrossRefPubMedPubMedCentral

Title: Diagnostic accuracy of the InBiOS AMD rapid diagnostic test for the detection of Burkholderia pseudomallei antigen in grown blood culture broth
Authors: Marjan Peeters
Panha Chung
Hua Lin
Kristien Mortelmans
Chhundy Phe
Chentha San
Laura Maria Francisca Kuijpers
Syna Teav
Thong Phe
Jan Jacobs
Publication date: 01-06-2018
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 6/2018
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-018-3237-3

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 6/2018

Associations between biomarkers at discharge and co-morbidities and risk of readmission after community-acquired pneumonia: a retrospective cohort study

Palivizumab prophylaxis for respiratory syncytial virus in infants with cystic fibrosis: is there a need?

Susceptibility profile of echinocandins, azoles and amphotericin B against yeast phase of Talaromyces marneffei isolated from HIV-infected patients in Guangdong, China

Influence of different peritoneal dialysis fluids on the in vitro activity of fosfomycin against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa

Increased overall survival after introduction of structured bedside consultation in Staphylococcus aureus bacteraemia

Epididymo-orchitis caused by enteric organisms in men > 35 years old: beyond fluoroquinolones

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials