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Diabetologia
Published in: Diabetologia 2/2005

01-02-2005 | Article

Dexamethasone impairs muscle energetics, studied by 31P NMR, in rats

Authors: J.-F. Dumas, G. Bielicki, J.-P. Renou, D. Roussel, P.-H. Ducluzeau, Y. Malthièry, G. Simard, P. Ritz

Published in: Diabetologia | Issue 2/2005

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Abstract

Aims/hypothesis

Glucocorticoid treatments are associated with increased whole-body oxygen consumption. We hypothesised that an impairment of muscle energy metabolism can participate in this increased energy expenditure.

Methods

To investigate this possibility, we have studied muscle energetics of dexamethasone-treated rats (1.5 mg kg−1 day−1 for 6 days), in vivo by 31P NMR spectroscopy. Results were compared with control and pair-fed (PF) rats before and after overnight fasting.

Results

Dexamethasone treatment resulted in decreased phosphocreatine (PCr) concentration and PCr:ATP ratio, increased ADP concentration and higher PCr to γ-ATP flux but no change in β-ATP to β-ADP flux in gastrocnemius muscle. Neither 4 days of food restriction (PF rats) nor 24 h fasting affected high-energy phosphate metabolism. In dexamethasone-treated rats, there was an increase in plasma insulin and non-esterified fatty acid concentration.

Conclusions/interpretation

We conclude that dexamethasone treatment altered resting in vivo skeletal muscle energy metabolism, by decreasing oxidative phosphorylation, producing ATP at the expense of PCr.
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Metadata
Title
Dexamethasone impairs muscle energetics, studied by 31P NMR, in rats
Authors
J.-F. Dumas
G. Bielicki
J.-P. Renou
D. Roussel
P.-H. Ducluzeau
Y. Malthièry
G. Simard
P. Ritz
Publication date
01-02-2005
Publisher
Springer-Verlag
Published in
Diabetologia / Issue 2/2005
Print ISSN: 0012-186X
Electronic ISSN: 1432-0428
DOI
https://doi.org/10.1007/s00125-004-1631-0

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