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01-03-2018 | Original Article

Developmental validation of a 6-dye STR kit with 27 loci

Authors: Shuanglin Li, Ling Chen, Yanfang Wang, Quyi Xu, Hong Liu, Yue Li, Chao Liu

Published in: International Journal of Legal Medicine | Issue 2/2018

Abstract

The EX27 System is a 6-dye (i.e., FAM, HEX, TAMAR, ROX, VIG, and SIZ-500) kit that combines the 24 autosomal STR loci (i.e., D3S1358, D13S317, D7S820, D16S539, D1S1656, Penta E, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391, FGA, D22S1045, D2S441, SE33) with a Y-STR locus (DYS391), an Indel site (YIndel) and Amelogenin, which is designed to amplify DNA from extracts as well as direct amplification from substrates. The new system contains most of the widely used autosomal STR loci around the world and is sensitive to the male component in mixtures and is more accurate in gender determination owing to the DYS391 and YIndel.

To demonstrate the suitability for personal identification and parentage testing, the system was validated for sensitivity of detection, species specificity, inhibitor tolerance, DNA mixtures, reproducibility study, and concordance studies based on the SWGDAM developmental validation guidelines. In view of the results, the EX27 System is a robust and reliable amplification kit which can be used for human identification testing.

Available only for authorised users

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Title: Developmental validation of a 6-dye STR kit with 27 loci
Authors: Shuanglin Li
Ling Chen
Yanfang Wang
Quyi Xu
Hong Liu
Yue Li
Chao Liu
Publication date: 01-03-2018
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 2/2018
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-017-1586-6

At a glance: The STEP trials

Springer Medicine

Developmental validation of a 6-dye STR kit with 27 loci

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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