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Open Access 01-04-2014 | Original Paper

Developmental Upregulation of an Alternative Form of pcp2 with Reduced GDI Activity

Authors: Jaroslaw J. Barski, Brad M. Denker, Jiazhen Guan, Matthias Lauth, Fabio Spreafico, Andrzej Fertala, Michael Meyer

Published in: The Cerebellum | Issue 2/2014

Abstract

The pcp2/L7 gene is characterized by its very cell type-specific expression restricted to cerebellar Purkinje cells and retinal bipolar neurons. Although remarkable progress as to the biochemical properties of the encoded protein has been made, knowledge on its physiological functions remains sparse. While characterizing a pcp2-driven transgenic strain, we observed the presence of a longer, so far unknown, pcp2 transcript. Different from another recently discovered splice variant, ret-pcp2, expression of this novel transcript is observed in bipolar as well as cerebellar Purkinje cells of mid-postnatal mice. The protein encoded by our novel variant appears to be less efficient in binding to Gα subunits compared to the original L7/pcp2 protein and it is also less inhibitory with respect to GTPγ binding. Its expression in the eye appears to be independent from eye opening in postnatal mice.

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Title: Developmental Upregulation of an Alternative Form of pcp2 with Reduced GDI Activity
Authors: Jaroslaw J. Barski
Brad M. Denker
Jiazhen Guan
Matthias Lauth
Fabio Spreafico
Andrzej Fertala
Michael Meyer
Publication date: 01-04-2014
Publisher: Springer US
Published in: The Cerebellum / Issue 2/2014
Print ISSN: 1473-4222
Electronic ISSN: 1473-4230
DOI: https://doi.org/10.1007/s12311-013-0529-0

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Developmental Upregulation of an Alternative Form of pcp2 with Reduced GDI Activity

Abstract

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