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Published in: Journal of Assisted Reproduction and Genetics 12/2016

01-12-2016 | Assisted Reproduction Technologies

Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection

Authors: Yamato Mizobe, Naoto Oya, Reiko Iwakiri, Naomi Yoshida, Yumi Sato, Nanase Onoue, Kazuchika Miyoshi, Makoto Tokunaga, Yuji Ezono

Published in: Journal of Assisted Reproduction and Genetics | Issue 12/2016

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Abstract

Purpose

In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.

Methods

Oocytes were divided into three groups—normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.

Results

The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7–67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).

Conclusions

The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.
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Metadata
Title
Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection
Authors
Yamato Mizobe
Naoto Oya
Reiko Iwakiri
Naomi Yoshida
Yumi Sato
Nanase Onoue
Kazuchika Miyoshi
Makoto Tokunaga
Yuji Ezono
Publication date
01-12-2016
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 12/2016
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-016-0811-4

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