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Published in: International Journal of Legal Medicine 3/2008

01-05-2008 | Short Communication

Development of a five ChX STRs loci typing system

Authors: Qiu-ling Liu, De-jian Lv, Xiang-lin Wu, Hong-yu Sun, Xin-yao Wu, Hui-ling Lu

Published in: International Journal of Legal Medicine | Issue 3/2008

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Abstract

We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up. A total of 827 unrelated individuals of the Han nationality in China were tested. The results show that the five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing, particularly for difficult paternity-deficient cases.
Literature
1.
go back to reference Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E, Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74PubMed Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E, Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74PubMed
2.
go back to reference Edelmann J, Deichsel D, Plate I, Kaser M, Szibor R (2003) Validation of the X-chromosomal STR DXS6809. Int J Legal Med 117:241–244PubMedCrossRef Edelmann J, Deichsel D, Plate I, Kaser M, Szibor R (2003) Validation of the X-chromosomal STR DXS6809. Int J Legal Med 117:241–244PubMedCrossRef
4.
go back to reference Zalan A, Volgyi A, Jung M, Peterman O, Pamjav H (2005) Hungarian population data of four X-linked markers: DXS8378, DXS7132, HPRTB, and DXS7423. Int J Legal Med 119:1–4CrossRef Zalan A, Volgyi A, Jung M, Peterman O, Pamjav H (2005) Hungarian population data of four X-linked markers: DXS8378, DXS7132, HPRTB, and DXS7423. Int J Legal Med 119:1–4CrossRef
5.
go back to reference Zarrabeitia MTAA, Martin J et al (2006) Study of six X-linked tetranucleotide microsatellites population. Int J Legal Med 120:147–150PubMedCrossRef Zarrabeitia MTAA, Martin J et al (2006) Study of six X-linked tetranucleotide microsatellites population. Int J Legal Med 120:147–150PubMedCrossRef
6.
go back to reference Szibor R, Lautsch S, Plate I, Beck N (2000) Population data on the X chromosome short tandem repeat locus HumHPRTB in two regions of Germany. J Forensic Sci 45:231–233PubMed Szibor R, Lautsch S, Plate I, Beck N (2000) Population data on the X chromosome short tandem repeat locus HumHPRTB in two regions of Germany. J Forensic Sci 45:231–233PubMed
7.
go back to reference Liu QL, Lv DJ, Zhu JZ, Lu HL, Luo YM, Fang Q (2006) Fluorescent multiplex amplification of three X-STR loci. Acta Genet Sin 33:1053–1059PubMedCrossRef Liu QL, Lv DJ, Zhu JZ, Lu HL, Luo YM, Fang Q (2006) Fluorescent multiplex amplification of three X-STR loci. Acta Genet Sin 33:1053–1059PubMedCrossRef
8.
go back to reference Shin SH, Yu JS, Park SW, Min GS, Chung KW (2005) Genetic analysis of 18 X-linked short tandem repeat markers in Korean population. Forensic Sci Int 147:35–41PubMedCrossRef Shin SH, Yu JS, Park SW, Min GS, Chung KW (2005) Genetic analysis of 18 X-linked short tandem repeat markers in Korean population. Forensic Sci Int 147:35–41PubMedCrossRef
9.
go back to reference Zarrabeitia MT, Amigo T, Sanudo C, Zarrabeitia A, Gonzalez-Lamuno D, Riancho JA (2002) A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome. Forensic Sci Int 129:85–89PubMedCrossRef Zarrabeitia MT, Amigo T, Sanudo C, Zarrabeitia A, Gonzalez-Lamuno D, Riancho JA (2002) A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome. Forensic Sci Int 129:85–89PubMedCrossRef
10.
go back to reference Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513PubMed Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513PubMed
11.
go back to reference Zaykin D, Zhivotovsky L, Weir BS (1995) Exact test for association between alleles at arbitrary number of loci. Genetica 96:169–178PubMedCrossRef Zaykin D, Zhivotovsky L, Weir BS (1995) Exact test for association between alleles at arbitrary number of loci. Genetica 96:169–178PubMedCrossRef
12.
go back to reference Desmarais D, Zhong Y, Chakraborty R, Perreault C, Busque L (1998) Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA). J Forensic Sci 43:1046–1049PubMed Desmarais D, Zhong Y, Chakraborty R, Perreault C, Busque L (1998) Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA). J Forensic Sci 43:1046–1049PubMed
13.
go back to reference Szibor R, Edelmann J, Hering S, Plate I, Wittig H, Roewer L, Wiegand P, Cali F, Romano V, Michael M (2003) Cell line DNA typing in forensic genetics—the necessity of reliable standards. Forensic Sci Int 138:37–43PubMedCrossRef Szibor R, Edelmann J, Hering S, Plate I, Wittig H, Roewer L, Wiegand P, Cali F, Romano V, Michael M (2003) Cell line DNA typing in forensic genetics—the necessity of reliable standards. Forensic Sci Int 138:37–43PubMedCrossRef
14.
go back to reference Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801-DXS6809-DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369PubMedCrossRef Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801-DXS6809-DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369PubMedCrossRef
Metadata
Title
Development of a five ChX STRs loci typing system
Authors
Qiu-ling Liu
De-jian Lv
Xiang-lin Wu
Hong-yu Sun
Xin-yao Wu
Hui-ling Lu
Publication date
01-05-2008
Publisher
Springer-Verlag
Published in
International Journal of Legal Medicine / Issue 3/2008
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-008-0223-9

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