Top

Published in:

01-07-2011 | Short Communication

Determination of acephate and methamidophos in tissues: appearance of matrix effect in gas chromatography–mass spectrometry

Authors: Nobuyuki Adachi, Hiroshi Kinoshita, Minori Nishiguchi, Motonori Takahashi, Harumi Ouchi, Takako Minami, Kiyoshi Matsui, Takehiko Yamamura, Shie Yoshida, Hajime Nishio

Published in: Forensic Toxicology | Issue 2/2011

Abstract

A simple and reliable method to determine acephate and methamidophos in mammalian tissues is presented. The method includes solid-phase extraction of tissue extracts with active carbon cartridges followed by gas chromatography–mass spectrometry analysis. During the study, a matrix effect was observed especially at low concentrations of acephate and methamidophos in serum and in brain. To minimize the effect, we prepared calibration curves with relatively short ranges. The validation data, such as the linearity of calibration curves, limits of detection, and coefficients of variation for recovery rates, were generally satisfactory. The present method is useful for determination of acephate and methamidophos in mammalian tissues because of its simplicity and speed, keeping in mind the presence of the matrix effect.

Tomlin CDS (ed) (1997) The pesticide manual, 11th edn. British Crop Protection Council, Surrey, pp 7–8, 808–809

Inoue S, Saito T, Miyazawa T, Mase H, Inokuchi S (2009) A simple method for detecting fenitrothion, its metabolite 3-methyl-4-nitrophenol, and other organophosphorus pesticides in human urine by LC-MS. Forensic Toxicol 27:32–36CrossRef

Kudo K, Ishida T, Hikiji W, Usumoto Y, Umehara T, Nagamatsu K, Tsuji A, Ikeda N (2010) Pattern of poisoning in Japan: selection of drugs and poisons for systematic toxicological analysis. Forensic Toxicol 28:25–32CrossRef

Adachi N, Kinoshita H, Nishiguchi M, Takahashi M, Ouchi H, Minami T, Matsui K, Yamamura T, Ohtsu N, Yoshida S, Hishida S (2008) Simultaneous analysis of acephate and methamidophos in human serum by improved extraction and GC–MS. Forensic Toxicol 26:76–79CrossRef

Russo MV, Campanella L, Avino P (2002) Determination of organophosphorus pesticide residues in human tissues by capillary gas chromatography–negative chemical ionization mass spectrometry analysis. J Chromatogr B 780:431–441CrossRef

Niessen WMA, Manini P, Andreoli R (2006) Matrix effects in quantitative pesticide analysis using liquid chromatography–mass spectrometry. Mass Spectrom Rev 25:881–899PubMedCrossRef

Kruve A, Künnapas A, Herodes K, Leito I (2008) Matrix effects in pesticide multi-residue analysis by liquid chromatography–mass spectrometry. J Chromatogr A 1187:58–66PubMedCrossRef

Ismaiel OA, Zhang T, Jenkins RG, Karnes HT (2010) Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry. J Chromatogr B 878:3303–3316CrossRef

Albero B, Sánchez-Brunete C, Tadeo JL (2005) Multiresidue determination of pesticides in juice by solid-phase extraction and gas chromatography–mass spectrometry. Talanta 66:917–924PubMedCrossRef

10.

Sánchez-Brunete C, Albero B, Martín G, Tadeo JL (2005) Determination of pesticide residues by GC–MS using analyte protectants to counteract the matrix effect. Anal Sci 21:1291–1296PubMedCrossRef

Title: Determination of acephate and methamidophos in tissues: appearance of matrix effect in gas chromatography–mass spectrometry
Authors: Nobuyuki Adachi
Hiroshi Kinoshita
Minori Nishiguchi
Motonori Takahashi
Harumi Ouchi
Takako Minami
Kiyoshi Matsui
Takehiko Yamamura
Shie Yoshida
Hajime Nishio
Publication date: 01-07-2011
Publisher: Springer Japan
Published in: Forensic Toxicology / Issue 2/2011
Print ISSN: 1860-8965
Electronic ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-011-0110-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Determination of acephate and methamidophos in tissues: appearance of matrix effect in gas chromatography–mass spectrometry

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2011

Identification and quantitation of two benzoylindoles AM-694 and (4-methoxyphenyl)(1-pentyl-1H-indol-3-yl)methanone, and three cannabimimetic naphthoylindoles JWH-210, JWH-122, and JWH-019 as adulterants in illegal products obtained via the Internet

Recently abused β-keto derivatives of 3,4-methylenedioxyphenylalkylamines: a review of their metabolisms and toxicological analysis

Imaging of methamphetamine incorporated into hair by MALDI-TOF mass spectrometry

Oripavine as a new marker of opiate product use

A model system for prediction of the in vivo metabolism of designer drugs using three-dimensional culture of rat and human hepatocytes

Mushroom toxins: a forensic toxicological review