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01-01-2016 | Original Article

Detection of cervical lesions by multivariate analysis of diffuse reflectance spectra: a clinical study

Authors: Vasumathi Gopala Prabitha, Sambasivan Suchetha, Jayaraj Lalitha Jayanthi, Kamalasanan Vijayakumary Baiju, Prabhakaran Rema, Koyippurath Anuraj, Anita Mathews, Paul Sebastian, Narayanan Subhash

Published in: Lasers in Medical Science | Issue 1/2016

Abstract

Diffuse reflectance (DR) spectroscopy is a non-invasive, real-time, and cost-effective tool for early detection of malignant changes in squamous epithelial tissues. The present study aims to evaluate the diagnostic power of diffuse reflectance spectroscopy for non-invasive discrimination of cervical lesions in vivo. A clinical trial was carried out on 48 sites in 34 patients by recording DR spectra using a point-monitoring device with white light illumination. The acquired data were analyzed and classified using multivariate statistical analysis based on principal component analysis (PCA) and linear discriminant analysis (LDA). Diagnostic accuracies were validated using random number generators. The receiver operating characteristic (ROC) curves were plotted for evaluating the discriminating power of the proposed statistical technique. An algorithm was developed and used to classify non-diseased (normal) from diseased sites (abnormal) with a sensitivity of 72 % and specificity of 87 %. While low-grade squamous intraepithelial lesion (LSIL) could be discriminated from normal with a sensitivity of 56 % and specificity of 80 %, and high-grade squamous intraepithelial lesion (HSIL) from normal with a sensitivity of 89 % and specificity of 97 %, LSIL could be discriminated from HSIL with 100 % sensitivity and specificity. The areas under the ROC curves were 0.993 (95 % confidence interval (CI) 0.0 to 1) and 1 (95 % CI 1) for the discrimination of HSIL from normal and HSIL from LSIL, respectively. The results of the study show that DR spectroscopy could be used along with multivariate analytical techniques as a non-invasive technique to monitor cervical disease status in real time.

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Title: Detection of cervical lesions by multivariate analysis of diffuse reflectance spectra: a clinical study
Authors: Vasumathi Gopala Prabitha
Sambasivan Suchetha
Jayaraj Lalitha Jayanthi
Kamalasanan Vijayakumary Baiju
Prabhakaran Rema
Koyippurath Anuraj
Anita Mathews
Paul Sebastian
Narayanan Subhash
Publication date: 01-01-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 1/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1829-z

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Detection of cervical lesions by multivariate analysis of diffuse reflectance spectra: a clinical study

Abstract

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Abstract

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