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BMC Cancer
Published in: BMC Cancer 1/2012

Open Access 01-12-2012 | Research article

Derivation and characterization of matched cell lines from primary and recurrent serous ovarian cancer

Authors: Isabelle J Létourneau, Michael CJ Quinn, Lu-Lin Wang, Lise Portelance, Katia Y Caceres, Louis Cyr, Nathalie Delvoye, Liliane Meunier, Manon de Ladurantaye, Zhen Shen, Suzanna L Arcand, Patricia N Tonin, Diane M Provencher, Anne-Marie Mes-Masson

Published in: BMC Cancer | Issue 1/2012

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Abstract

Background

Cell line models have proven to be effective tools to investigate a variety of ovarian cancer features. Due to the limited number of cell lines, particularly of the serous subtype, the heterogeneity of the disease, and the lack of cell lines that model disease progression, there is a need to further develop cell line resources available for research. This study describes nine cell lines derived from three ovarian cancer cases that were established at initial diagnosis and at subsequent relapse after chemotherapy.

Methods

The cell lines from three women diagnosed with high-grade serous ovarian cancer (1369, 2295 and 3133) were derived from solid tumor (TOV) and ascites (OV), at specific time points at diagnosis and relapse (R). Primary treatment was a combination of paclitaxel/carboplatin (1369, 3133), or cisplatin/topotecan (2295). Second line treatment included doxorubicin, gemcitabine and topotecan. In addition to molecular characterization (p53, HER2), the cell lines were characterized based on cell growth characteristics including spheroid growth, migration potential, and anchorage independence. The in vivo tumorigenicity potential of the cell lines was measured. Response to paclitaxel and carboplatin was assessed using a clonogenic assay.

Results

All cell lines had either a nonsense or missense TP53 mutations. The ability to form compact spheroids or aggregates was observed in six of nine cell lines. Limited ability for migration and anchorage independence was observed. The OV3133(R) cell line, formed tumors at subcutaneous sites in SCID mice. Based on IC50 values and dose response curves, there was clear evidence of acquired resistance to carboplatin for TOV2295(R) and OV2295(R2) cell lines.

Conclusion

The study identified nine new high-grade serous ovarian cancer cell lines, derived before and after chemotherapy that provides a unique resource for investigating the evolution of this common histopathological subtype of ovarian cancer.
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Metadata
Title
Derivation and characterization of matched cell lines from primary and recurrent serous ovarian cancer
Authors
Isabelle J Létourneau
Michael CJ Quinn
Lu-Lin Wang
Lise Portelance
Katia Y Caceres
Louis Cyr
Nathalie Delvoye
Liliane Meunier
Manon de Ladurantaye
Zhen Shen
Suzanna L Arcand
Patricia N Tonin
Diane M Provencher
Anne-Marie Mes-Masson
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2012
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-12-379

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