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Published in: Clinical Oral Investigations 4/2019

01-04-2019 | Original Article

Dentinal tubule penetration of AH Plus, BC Sealer and a novel tricalcium silicate sealer: a confocal laser scanning microscopy study

Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Maha Daou, Nada El Osta, Alfred Naaman, Marie Abboud

Published in: Clinical Oral Investigations | Issue 4/2019

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Abstract

Objectives

The aim of this in vitro study was to assess the dentinal tubule penetration of three different sealers, AH Plus, BC Sealer and a novel tricalcium silicate sealer (NTS).

Materials and methods

Ninety-six human maxillary central incisors were divided into three experimental groups (n = 32) and were filled with gutta-percha using a single-cone technique in conjunction with one of the three sealers: AH Plus, BC Sealer or NTS. The roots in each group were cross-sectioned at 1 and 5 mm from the root apex, and the surfaces were examined under confocal laser scanning microscopy (CLSM). The sealer penetration depths were measured at their maximum depths and at four circumferential depths (12, 3, 6 and 9 o’clock) and were evaluated using ImageJ software (ImageJ, NIH).

Results

The maximum and mean penetration depths were significantly higher at 5 mm compared to 1 mm from the apex in the AH Plus (p < 0.001), BC Sealer (p < 0.001) and NTS groups (p < 0.001). No significant difference was observed between the groups at 1 mm for both parameters. The maximum and mean penetration depths were significantly lower at 5 mm for AH Plus compared with the other two groups (p = 0.012).

Conclusions

Within the study limitations, the BC Sealer and NTS demonstrated better tubule penetration results than the AH Plus sealer.

Clinical relevance

Although no study has confirmed a relationship between the penetration depth of root canal sealers and the prevention of apical periodontitis, dentinal tubule sealer penetration may improve obturation quality.
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Metadata
Title
Dentinal tubule penetration of AH Plus, BC Sealer and a novel tricalcium silicate sealer: a confocal laser scanning microscopy study
Authors
Roula El Hachem
Issam Khalil
Guy Le Brun
Fabrice Pellen
Bernard Le Jeune
Maha Daou
Nada El Osta
Alfred Naaman
Marie Abboud
Publication date
01-04-2019
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 4/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-018-2632-6

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