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Open Access 01-12-2015 | Research

Dental follicle mesenchymal stem cell administration ameliorates muscle weakness in MuSK-immunized mice

Authors: Canan Ulusoy, Noushin Zibandeh, Selin Yıldırım, Nikolaos Trakas, Paraskevi Zisimopoulou, Melike Küçükerden, Hatice Tașlı, Socrates Tzartos, Kamil Göker, Erdem Tüzün, Tunç Akkoç

Published in: Journal of Neuroinflammation | Issue 1/2015

Abstract

Background

Myasthenia gravis (MG) is an antibody-mediated autoimmune disease of the neuromuscular junction (NMJ), mostly associated with acetylcholine receptor (AChR) antibodies. Around 5–10 % of MG patients show antibodies to muscle-specific tyrosine kinase (MuSK). Mesenchymal stem cell (MSC) administration has been shown to ameliorate muscle weakness in the experimental autoimmune myasthenia gravis (EAMG) model induced by AChR immunization.

Methods

To investigate the efficacy of stem cell treatment in MuSK-related EAMG, clinical and immunological features of MuSK-immunized mice with or without dental follicle MSC (DFMSC) treatment were compared.

Results

MuSK-immunized mice intravenously treated with DFMSC after second and third immunizations showed significantly lower EAMG incidence and severity and reduced serum anti-MuSK antibody, NMJ IgG, and C3 deposit levels and CD11b+ lymph node cell ratios. Moreover, lymph node cells of DFMSC-administered mice showed reduced proliferation and IL-6 and IL-12 production responses to MuSK stimulation. By contrast, proportions of B and T cell populations and production of a wide variety of cytokines were not affected from DFMSC treatment.

Conclusions

Our results suggest that DFMSC treatment shows its beneficial effects mostly through suppression of innate immune system, whereas other immune functions appear to be preserved. Stem cell treatment might thus constitute a specific and effective treatment method in MuSK-associated MG.

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Title: Dental follicle mesenchymal stem cell administration ameliorates muscle weakness in MuSK-immunized mice
Authors: Canan Ulusoy
Noushin Zibandeh
Selin Yıldırım
Nikolaos Trakas
Paraskevi Zisimopoulou
Melike Küçükerden
Hatice Tașlı
Socrates Tzartos
Kamil Göker
Erdem Tüzün
Tunç Akkoç
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Journal of Neuroinflammation / Issue 1/2015
Electronic ISSN: 1742-2094
DOI: https://doi.org/10.1186/s12974-015-0451-0

At a glance: The STEP trials

Springer Medicine

Dental follicle mesenchymal stem cell administration ameliorates muscle weakness in MuSK-immunized mice

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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