Skip to main content
Top
Published in: Lasers in Medical Science 1/2009

01-01-2009 | Original Article

Decontamination of deep dentin by means of erbium, chromium:yttrium-scandium-gallium-garnet laser irradiation

Authors: René Franzen, Marcella Esteves-Oliveira, Jörg Meister, Anja Wallerang, Leon Vanweersch, Friedrich Lampert, Norbert Gutknecht

Published in: Lasers in Medical Science | Issue 1/2009

Login to get access

Abstract

The aim of this in vitro study was to evaluate the depth of effectiveness of erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation on microorganism reduction. From human roots, dentin slices of 100 μm to 1,000 μm thickness were prepared. These specimens were sterilized and then inoculated with 1 μl of Enterococcus faecalis suspension. The backs of the specimens were then irradiated with Er,Cr:YSGG radiation at a pulse energy of 3.13 mJ, delivered at an incidence angle of 5° to the dentin slice surface. A control group was left without irradiation. The remaining bacteria were collected in 1 ml sterilized NaCl solution, serially diluted and seeded in Columbia-Agar plates. Despite the low pulse energy of 3.13 mJ, the Er,Cr:YSGG laser irradiation resulted in significant bacterial reduction up to a dentin thickness of 500 μm (P < 0.05). Scanning electron microscopy (SEM) micrographs of the contaminated and irradiated surfaces showed the absence of a smear layer and opened dentinal tubules.
Literature
1.
go back to reference Mehl A, Folwaczny M, Haffner C, Hickel R (1999) Bactericidal effects of 2.94 microns Er:YAG-laser radiation in dental root canals. J Endod 25:490–493PubMedCrossRef Mehl A, Folwaczny M, Haffner C, Hickel R (1999) Bactericidal effects of 2.94 microns Er:YAG-laser radiation in dental root canals. J Endod 25:490–493PubMedCrossRef
2.
go back to reference Berkiten M, Berkiten R, Okar I (2000) Comparative evaluation of antibacterial effects of Nd:YAG laser irradiation in root canals and dentinal tubules. J Endod 26:268–270PubMedCrossRef Berkiten M, Berkiten R, Okar I (2000) Comparative evaluation of antibacterial effects of Nd:YAG laser irradiation in root canals and dentinal tubules. J Endod 26:268–270PubMedCrossRef
3.
go back to reference Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F (2000) Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg 18:57–60PubMed Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F (2000) Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg 18:57–60PubMed
4.
go back to reference Klinke T, Klimm W, Gutknecht N (1997) Antibacterial effects of Nd:YAG laser irradiation within root canal dentin. J Clin Laser Med Surg 15:29–31PubMed Klinke T, Klimm W, Gutknecht N (1997) Antibacterial effects of Nd:YAG laser irradiation within root canal dentin. J Clin Laser Med Surg 15:29–31PubMed
5.
go back to reference Piccolomini R, D’Arcangelo C, D’Ercole S, Catamo G, Schiaffino G, De Fazio P (2002) Bacteriologic evaluation of the effect of Nd:YAG laser irradiation in experimental infected root canals. J Endod 28:276–278PubMedCrossRef Piccolomini R, D’Arcangelo C, D’Ercole S, Catamo G, Schiaffino G, De Fazio P (2002) Bacteriologic evaluation of the effect of Nd:YAG laser irradiation in experimental infected root canals. J Endod 28:276–278PubMedCrossRef
6.
go back to reference Moritz A, Gutknecht N, Schoop U, Goharkhay K, Doertbudak O, Sperr W (1997) Irradiation of infected root canals with a diode laser in vivo: results of microbiological examinations. Lasers Surg Med 21:221–226PubMedCrossRef Moritz A, Gutknecht N, Schoop U, Goharkhay K, Doertbudak O, Sperr W (1997) Irradiation of infected root canals with a diode laser in vivo: results of microbiological examinations. Lasers Surg Med 21:221–226PubMedCrossRef
7.
go back to reference Le Goff A, Dautel-Morazin A, Guigand M, Vulcain JM, Bonnaure-Mallet M (1999) An evaluation of the CO2 laser for endodontic disinfection. J Endod 25:105–108PubMedCrossRef Le Goff A, Dautel-Morazin A, Guigand M, Vulcain JM, Bonnaure-Mallet M (1999) An evaluation of the CO2 laser for endodontic disinfection. J Endod 25:105–108PubMedCrossRef
8.
go back to reference Gutknecht N, Nuebler-Moritz M, Burghardt SF, Lampert F (1997) The efficiency of root canal disinfection using a holmium:yttrium-aluminum-garnet laser in vitro. J Clin Laser Med Surg 15:75–78PubMed Gutknecht N, Nuebler-Moritz M, Burghardt SF, Lampert F (1997) The efficiency of root canal disinfection using a holmium:yttrium-aluminum-garnet laser in vitro. J Clin Laser Med Surg 15:75–78PubMed
9.
go back to reference Folwaczny M, Liesenhoff T, Lehn N, Horch HH (1998) Bactericidal action of 308 nm excimer-laser radiation: an in vitro investigation. J Endod 24:781–785PubMedCrossRef Folwaczny M, Liesenhoff T, Lehn N, Horch HH (1998) Bactericidal action of 308 nm excimer-laser radiation: an in vitro investigation. J Endod 24:781–785PubMedCrossRef
10.
go back to reference Moritz A, Schoop U, Goharkhay K, Jakolitsch S, Kluger W, Wernisch J, Sperr W (1999) The bactericidal effect of Nd:YAG, Ho:YAG, and Er:YAG laser irradiation in the root canal: an in vitro comparison. J Clin Laser Med Surg 17:161–164PubMed Moritz A, Schoop U, Goharkhay K, Jakolitsch S, Kluger W, Wernisch J, Sperr W (1999) The bactericidal effect of Nd:YAG, Ho:YAG, and Er:YAG laser irradiation in the root canal: an in vitro comparison. J Clin Laser Med Surg 17:161–164PubMed
11.
go back to reference Folwaczny M, Mehl A, Jordan C, Hickel R (2002) Antibacterial effects of pulsed Nd:YAG laser radiation at different energy settings in root canals. J Endod 28:24–29PubMedCrossRef Folwaczny M, Mehl A, Jordan C, Hickel R (2002) Antibacterial effects of pulsed Nd:YAG laser radiation at different energy settings in root canals. J Endod 28:24–29PubMedCrossRef
13.
go back to reference Gutknecht N, Kaiser F, Hassan A, Lampert F (1996) Long-term clinical evaluation of endodontically treated teeth by Nd:YAG lasers. J Clin Laser Med Surg 14:7–11PubMed Gutknecht N, Kaiser F, Hassan A, Lampert F (1996) Long-term clinical evaluation of endodontically treated teeth by Nd:YAG lasers. J Clin Laser Med Surg 14:7–11PubMed
14.
go back to reference Gutknecht N, Moritz A, Conrads G, Sievert T, Lampert F (1996) Bactericidal effect of the Nd:YAG laser in in vitro root canals. J Clin Laser Med Surg 14:77–80PubMed Gutknecht N, Moritz A, Conrads G, Sievert T, Lampert F (1996) Bactericidal effect of the Nd:YAG laser in in vitro root canals. J Clin Laser Med Surg 14:77–80PubMed
15.
go back to reference Moritz A, Doertbudak O, Gutknecht N, Goharkhay K, Schoop U, Sperr W (1997) Nd:YAG laser irradiation of infected root canals in combination with microbiological examinations. J Am Dent Assoc 128:1525–1530PubMed Moritz A, Doertbudak O, Gutknecht N, Goharkhay K, Schoop U, Sperr W (1997) Nd:YAG laser irradiation of infected root canals in combination with microbiological examinations. J Am Dent Assoc 128:1525–1530PubMed
16.
go back to reference Gutknecht N, Franzen R, Schippers M, Lampert F (2004) Bactericidal effect of a 980-nm diode laser in the root canal wall dentin of bovine teeth. J Clin Laser Med Surg 22:9–13PubMedCrossRef Gutknecht N, Franzen R, Schippers M, Lampert F (2004) Bactericidal effect of a 980-nm diode laser in the root canal wall dentin of bovine teeth. J Clin Laser Med Surg 22:9–13PubMedCrossRef
17.
go back to reference Gouw-Soares S, Gutknecht N, Conrads G, Lampert F, Matson E, Eduardo CP (2000) The bactericidal effect of Ho:YAG laser irradiation within contaminated root dentinal samples. J Clin Laser Med Surg 18:81–87PubMed Gouw-Soares S, Gutknecht N, Conrads G, Lampert F, Matson E, Eduardo CP (2000) The bactericidal effect of Ho:YAG laser irradiation within contaminated root dentinal samples. J Clin Laser Med Surg 18:81–87PubMed
18.
go back to reference Takeda FH, Harashima T, Kimura Y, Matsumoto K (1998) Comparative study about the removal of smear layer by three types of laser devices. J Clin Laser Med Surg 16:117–122PubMed Takeda FH, Harashima T, Kimura Y, Matsumoto K (1998) Comparative study about the removal of smear layer by three types of laser devices. J Clin Laser Med Surg 16:117–122PubMed
19.
go back to reference Gutknecht N, Behren VG (1991) The Nd:YAG laser as an aid to root canal obturation. In: Vogel G (ed) Monduzzi Editore, Milan, pp I275–I280 Gutknecht N, Behren VG (1991) The Nd:YAG laser as an aid to root canal obturation. In: Vogel G (ed) Monduzzi Editore, Milan, pp I275–I280
20.
go back to reference Santos C, Sousa-Neto MD, Alfredo E, Guerisoli DM, Pecora JD, Comelli Lia RF (2005) Morphologic evaluation of the radicular dentine irradiated with Nd:YAG laser under different parameters and angles of incidence. Photomed Laser Surg 23:590–595PubMedCrossRef Santos C, Sousa-Neto MD, Alfredo E, Guerisoli DM, Pecora JD, Comelli Lia RF (2005) Morphologic evaluation of the radicular dentine irradiated with Nd:YAG laser under different parameters and angles of incidence. Photomed Laser Surg 23:590–595PubMedCrossRef
21.
go back to reference Ramalho KM, Marques MM, Apel C, Meneguzzo DT, Eduardo CP (2005) Morphological analysis of root canal walls after Er:YAG and Nd:YAG laser irradiation: a preliminary SEM investigation. J Oral Laser Appl 5:91–96 Ramalho KM, Marques MM, Apel C, Meneguzzo DT, Eduardo CP (2005) Morphological analysis of root canal walls after Er:YAG and Nd:YAG laser irradiation: a preliminary SEM investigation. J Oral Laser Appl 5:91–96
22.
go back to reference Harashima T, Takeda FH, Kimura Y, Matsumoto K (1997) Effect of Nd:YAG laser irradiation for removal of intracanal debris and smear layer in extracted human teeth. J Clin Laser Med Surg 15:131–135PubMed Harashima T, Takeda FH, Kimura Y, Matsumoto K (1997) Effect of Nd:YAG laser irradiation for removal of intracanal debris and smear layer in extracted human teeth. J Clin Laser Med Surg 15:131–135PubMed
23.
go back to reference Zezell DM, Cecchini SC, Eduardo CP, Matsumoto K, De Rossi W, Nogueira GE, Berretta JR, Vieira Junior ND, Morato SP (1995) Experimental studies of the applications of the holmium laser in dentistry. J Clin Laser Med Surg 13:283–289PubMed Zezell DM, Cecchini SC, Eduardo CP, Matsumoto K, De Rossi W, Nogueira GE, Berretta JR, Vieira Junior ND, Morato SP (1995) Experimental studies of the applications of the holmium laser in dentistry. J Clin Laser Med Surg 13:283–289PubMed
24.
go back to reference Takeda FH, Harashima T, Kimura Y, Matsumoto K (1998) Efficacy of Er:YAG laser irradiation in removing debris and smear layer on root canal walls. J Endod 24:548–551PubMedCrossRef Takeda FH, Harashima T, Kimura Y, Matsumoto K (1998) Efficacy of Er:YAG laser irradiation in removing debris and smear layer on root canal walls. J Endod 24:548–551PubMedCrossRef
25.
go back to reference Matsuoka E, Kimura Y, Matsumoto K (1998) Studies on the removal of debris near the apical seats by Er:YAG laser and assessment with a fiberscope. J Clin Laser Med Surg 16:255–261PubMed Matsuoka E, Kimura Y, Matsumoto K (1998) Studies on the removal of debris near the apical seats by Er:YAG laser and assessment with a fiberscope. J Clin Laser Med Surg 16:255–261PubMed
26.
go back to reference Theodoro LH, Haypek P, Bachmann L, Garcia VG, Sampaio JE, Zezell DM, Eduardo Cde P (2003) Effect of ER:YAG and diode laser irradiation on the root surface: morphological and thermal analysis. J Periodontol 74:838–843PubMedCrossRef Theodoro LH, Haypek P, Bachmann L, Garcia VG, Sampaio JE, Zezell DM, Eduardo Cde P (2003) Effect of ER:YAG and diode laser irradiation on the root surface: morphological and thermal analysis. J Periodontol 74:838–843PubMedCrossRef
27.
go back to reference Wang X, Sun Y, Kimura Y, Kinoshita J, Ishizaki NT, Matsumoto K (2005) Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed Laser Surg 23:575–581PubMedCrossRef Wang X, Sun Y, Kimura Y, Kinoshita J, Ishizaki NT, Matsumoto K (2005) Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed Laser Surg 23:575–581PubMedCrossRef
28.
go back to reference Yamazaki R, Goya C, Yu DG, Kimura Y, Matsumoto K (2001) Effects of erbium,chromium:YSGG laser irradiation on root canal walls: a scanning electron microscopic and thermographic study. J Endod 27:9–12PubMedCrossRef Yamazaki R, Goya C, Yu DG, Kimura Y, Matsumoto K (2001) Effects of erbium,chromium:YSGG laser irradiation on root canal walls: a scanning electron microscopic and thermographic study. J Endod 27:9–12PubMedCrossRef
29.
go back to reference Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W (2004) Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 35:111–116PubMedCrossRef Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W (2004) Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 35:111–116PubMedCrossRef
30.
go back to reference Rooney J, Midda M, Leeming J (1994) A laboratory investigation of the bactericidal effect of a NdYAG laser. Br Dent J 176:61–64PubMedCrossRef Rooney J, Midda M, Leeming J (1994) A laboratory investigation of the bactericidal effect of a NdYAG laser. Br Dent J 176:61–64PubMedCrossRef
31.
go back to reference Fabricius L, Dahlen G, Sundqvist G, Happonen RP, Moller AJ (2006) Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci 114:278–285PubMedCrossRef Fabricius L, Dahlen G, Sundqvist G, Happonen RP, Moller AJ (2006) Influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. Eur J Oral Sci 114:278–285PubMedCrossRef
32.
go back to reference Haapasalo M, Ranta H, Ranta KT (1983) Facultative gram-negative enteric rods in persistent periapical infections. Acta Odontol Scand 41:19–22PubMed Haapasalo M, Ranta H, Ranta KT (1983) Facultative gram-negative enteric rods in persistent periapical infections. Acta Odontol Scand 41:19–22PubMed
33.
go back to reference Haapasalo M, Orstavik D (1987) In vitro infection and disinfection of dentinal tubules. J Dent Res 66:1375–1379PubMed Haapasalo M, Orstavik D (1987) In vitro infection and disinfection of dentinal tubules. J Dent Res 66:1375–1379PubMed
34.
go back to reference Love RM (2001) Enterococcus faecalis—a mechanism for its role in endodontic failure. Int Endod J 34:399–405PubMedCrossRef Love RM (2001) Enterococcus faecalis—a mechanism for its role in endodontic failure. Int Endod J 34:399–405PubMedCrossRef
35.
go back to reference Kesler G, Gal R, Kesler A, Koren R (2002) Histological and scanning electron microscope examination of root canal after preparation with Er:YAG laser microprobe: a preliminary in vitro study. J Clin Laser Med Surg 20:269–277PubMedCrossRef Kesler G, Gal R, Kesler A, Koren R (2002) Histological and scanning electron microscope examination of root canal after preparation with Er:YAG laser microprobe: a preliminary in vitro study. J Clin Laser Med Surg 20:269–277PubMedCrossRef
36.
go back to reference Yu DG, Kimura Y, Kinoshita J, Matsumoto K (2000) Morphological and atomic analytical studies on enamel and dentin irradiated by an erbium, chromium:YSGG laser. J Clin Laser Med Surg 18:139–143PubMed Yu DG, Kimura Y, Kinoshita J, Matsumoto K (2000) Morphological and atomic analytical studies on enamel and dentin irradiated by an erbium, chromium:YSGG laser. J Clin Laser Med Surg 18:139–143PubMed
37.
go back to reference Hossain M, Nakamura Y, Yamada Y, Suzuki N, Murakami Y, Matsumoto K (2001) Analysis of surface roughness of enamel and dentin after Er,Cr:YSGG laser irradiation. J Clin Laser Med Surg 19:297–303PubMedCrossRef Hossain M, Nakamura Y, Yamada Y, Suzuki N, Murakami Y, Matsumoto K (2001) Analysis of surface roughness of enamel and dentin after Er,Cr:YSGG laser irradiation. J Clin Laser Med Surg 19:297–303PubMedCrossRef
38.
go back to reference Aoki A, Ishikawa I, Yamada T, Otsuki M, Watanabe H, Tagami J, Ando Y, Yamamoto H (1998) Comparison between Er:YAG laser and conventional technique for root caries treatment in vitro. J Dent Res 77:1404–1414PubMedCrossRef Aoki A, Ishikawa I, Yamada T, Otsuki M, Watanabe H, Tagami J, Ando Y, Yamamoto H (1998) Comparison between Er:YAG laser and conventional technique for root caries treatment in vitro. J Dent Res 77:1404–1414PubMedCrossRef
39.
go back to reference Kimura Y, Yu DG, Kinoshita J, Hossain M, Yokoyama K, Murakami Y, Nomura K, Takamura R, Matsumoto K (2001) Effects of erbium, chromium:YSGG laser irradiation on root surface: morphological and atomic analytical studies. J Clin Laser Med Surg 19:69–72PubMedCrossRef Kimura Y, Yu DG, Kinoshita J, Hossain M, Yokoyama K, Murakami Y, Nomura K, Takamura R, Matsumoto K (2001) Effects of erbium, chromium:YSGG laser irradiation on root surface: morphological and atomic analytical studies. J Clin Laser Med Surg 19:69–72PubMedCrossRef
40.
go back to reference Matsumoto K, Hossain M, Hossain MM, Kawano H, Kimura Y (2002) Clinical assessment of Er,Cr:YSGG laser application for cavity preparation. J Clin Laser Med Surg 20:17–21PubMedCrossRef Matsumoto K, Hossain M, Hossain MM, Kawano H, Kimura Y (2002) Clinical assessment of Er,Cr:YSGG laser application for cavity preparation. J Clin Laser Med Surg 20:17–21PubMedCrossRef
41.
go back to reference Kimura Y, Yu DG, Fujita A, Yamashita A, Murakami Y, Matsumoto K (2001) Effects of erbium,chromium:YSGG laser irradiation on canine mandibular bone. J Periodontol 72:1178–1182PubMedCrossRef Kimura Y, Yu DG, Fujita A, Yamashita A, Murakami Y, Matsumoto K (2001) Effects of erbium,chromium:YSGG laser irradiation on canine mandibular bone. J Periodontol 72:1178–1182PubMedCrossRef
Metadata
Title
Decontamination of deep dentin by means of erbium, chromium:yttrium-scandium-gallium-garnet laser irradiation
Authors
René Franzen
Marcella Esteves-Oliveira
Jörg Meister
Anja Wallerang
Leon Vanweersch
Friedrich Lampert
Norbert Gutknecht
Publication date
01-01-2009
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 1/2009
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-007-0522-2

Other articles of this Issue 1/2009

Lasers in Medical Science 1/2009 Go to the issue