Top

BMC Complementary Medicine and Therapies

Published in:

Open Access 01-12-2014 | Research article

Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1

Authors: Xiaoguang Lu, Xin Kang, Libin Zhan, Chunyu Lv, Zhiwei Fan, Yingli Wang, Robbie Ali, Chang Lv, Siyao Li, Jinhai Mu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

Abstract

Background

Dai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction, acute pancreatitis, cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying mechanism has not been fully elucidated.

Methods

SD male rats in adult were used to model HS and treated with DHFZT. The serum concentration of endotoxin were analyzed by dynamic turbidimetric method. In addition, the blood flow of small intestine were measured using laser speckle technique. Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 protein, intestinal fatty acid binding protein (IFABP) were measured by Western Blotting, RT-PCR, ELISA respectively.

Results

Present study showed that DHFZT markedly elevated the blood flow of small intestine, protected the intestinal barrier function by up-regulating the expression of ZO-1 protein and down-regulating expression of p-VASP, and notely decreased serum concentration of IFABP and endotoxin in HS.

Conclusions

These results reveal that DHFZT improves intestinal blood flow, protects the intestinal barrier function, and ameliorates intestinal endotoxaemia in rats with HS.

Available only for authorised users

Kauvar DS, Lefering R, Wade CE: Impact of hemorrhage on trauma outcome: an overview of epidemiology, clinical presentations, and therapeutic considerations. J Trauma. 2006, 60: S3-S11. 10.1097/01.ta.0000199961.02677.19.CrossRefPubMed

World Health Organization: World Health Statistics 2010. 2010, Geneva, Switzerland: World Health Organization Press, 62-70.

Wilson M, Davis DP, Coimbra R: Diagnosis and monitoring of hemorrhagic shock during the initial resuscitation of multiple trauma patients: a review. J Emerg Med. 2003, 24: 413-422. 10.1016/S0736-4679(03)00042-8.CrossRefPubMed

Korff S, Loughran P, Cai C, Lee YS, Scott M, Billiar TR: Eritoran attenuates tissue damage and inflammation in hemorrhagic shock/trauma. J Surg Res. 2013, 184: e17-25. 10.1016/j.jss.2013.03.023.CrossRefPubMed

Levy G, Fishman JE, Xu D, Chandler BT, Feketova E, Dong W, Qin Y, Alli V, Ulloa L, Deitch EA: Parasympathetic stimulation via the vagus nerve prevents systemic organ dysfunction by abrogating gut injury and lymph toxicity in trauma and hemorrhagic shock. Shock. 2013, 39: 39-44.PubMedPubMedCentral

Doucet D, Badami C, Palange D, Bonitz RP, Lu Q, Xu DZ, Kannan KB, Colorado I, Feinman R, Deitch EA: Estrogen receptor hormone agonists limit trauma hemorrhage shock-induced gut and lung injury in rats. PLoS One. 2010, 5: e9421-10.1371/journal.pone.0009421.CrossRefPubMedPubMedCentral

Lu XG, Zhan LB, Kang X, Liu L, Li YZ, Yu J, Fan ZW, Bai LZ, Ji CY, Wang XZ: Clinical research of Dahuang Fuzi decoction in auxiliary treatment of severe acute pancreatitis: a multi-center observation in 206 patients. Chin Crit Care Med. 2010, 22: 723-728.

Wang B, Cheng G, Lei FY: Treatment of 36 cases of acute appendicitis by double needle with Dai Huang Fu Zi Tang. Shai xi J Tradit Chin Med. 2006, 27: 723-724.

Jin YC, Shen HB: Dai Huang Fu Zi Tang treatment for 100 case report. J Hebei TCM Pharmacol. 2001, 16: 28-

10.

Xu QH: Early treatment of acute intra-abdominal hypertension by Dai Huang Fu Zi Tang. Chin Pri Health Care. 2012, 26: 111-112.

11.

Lu XG, Zhan LB, Kang X, Liu GH, Dong Y, Fan ZW, Bai LZ, Liu L, Ji CY, Wang XZ: Protective effects of Dahuangfuzi decoction on the intestine barrier functional of acute necrotizing pancreatitis in rats. Chin J Pancreatol. 2011, 11: 113-116.

12.

Li H, Guo H, Wu L, Zhang Y, Chen J, Liu X, Cai H, Zhang K, Cai B: Comparative pharmacokinetics study of three anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang by high performance liquid chromatography-mass spectrometry. J Pharm Biomed Anal. 2013, 76: 215-218.CrossRefPubMed

13.

Sulaberidze G, Ghvaladze M, Didava G, Kochlamazashvili B, Chkhaidze M, Gegeshidze T: Changes of redox processes in liver under pv-88 at experimental hemorrhagic shock. Georgian Med News. 2011, 190: 65-69.PubMed

14.

Xie F, Hou KD, Song Q, Jiang CG: The change in bone marrow after intraosseous hypertonic saline-hydroxyethyl starch infusion for resuscitation of hemorrhagic shock in dog. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010, 22: 309-312.PubMed

15.

Remmele W, Schicketanz KH: Immunohistochemical determination of estrogen and progesterone receptor content in human breast cancer. Computer-assisted image analysis (QIC score) vs subjective grading (IRS). Pathol Res Pract. 1993, 189: 227-245. 10.1016/S0344-0338(11)80100-7.CrossRef

16.

Chiu CJ, McArdle AH, Brown R, Scott HJ, Gurd FN: Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg. 1970, 101: 478-583. 10.1001/archsurg.1970.01340280030009.CrossRefPubMed

17.

Zakaria el R, Tsakadze NL, Garrison RN: Hypertonic saline resuscitation improves intestinal microcirculation in a rat model of hemorrhagic shock. Surgery. 2006, 140: 579-587. 10.1016/j.surg.2006.05.015.CrossRefPubMedPubMedCentral

18.

Hirota K, Turner JE, Villa M, Duarte JH, Demengeot J, Steinmetz OM, Stockinger B: Plasticity of Th17 cells in Peyer’s patches is responsible for the induction of T cell-dependent IgA responses. Nat Immunol. 2013, 14: 372-379. 10.1038/ni.2552.CrossRefPubMedPubMedCentral

19.

Adriaanse MP, Tack GJ, Passos VL, Damoiseaux JG, Schreurs MW, van Wijck K, Riedl RG, Masclee AA, Buurman WA, Mulder CJ, Vreugdenhil AC: Serum I-FABP as marker for enterocyte damage in coeliac disease and its relation to villous atrophy and circulating autoantibodies. Aliment Pharmacol Ther. 2013, 37: 482-490. 10.1111/apt.12194.CrossRefPubMed

20.

Vermeulen Windsant IC, Hellenthal FA, Derikx JP, Prins MH, Buurman WA, Jacobs MJ, Schurink GW: Circulating intestinal fatty acid-binding protein as an early marker of intestinal necrosis after aortic surgery: a prospective observational cohort study. Ann Surg. 2012, 255: 796-803. 10.1097/SLA.0b013e31824b1e16.CrossRefPubMed

21.

Guo S, Al-Sadi R, Said HM, Ma TY: Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization of TLR-4 and CD14. Am J Pathol. 2013, 182: 375-387. 10.1016/j.ajpath.2012.10.014.CrossRefPubMedPubMedCentral

22.

Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, Yashiro K, Tsukita S, Hamada H: EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins. Dev Biol. 2012, 371: 136-145. 10.1016/j.ydbio.2012.07.005.CrossRefPubMed

23.

Jouret F, Wu J, Hull M, Rajendran V, Mayr B, Schöfl C, Geibel J, Caplan MJ: Activation of the Ca²⁺-sensing receptor induces deposition of tight junction components to the epithelial cell plasma membrane. J Cell Sci. 2013, 126: 5132-5142. 10.1242/jcs.127555.CrossRefPubMedPubMedCentral

24.

Shen ZY, Zhang J, Song HL, Zheng WP: Bone-marrow mesenchymal stem cells reduce rat intestinal ischemia-reperfusion injury, ZO-1 downregulation and tight junction disruption via a TNF-α-regulated mechanism. World J Gastroenterol. 2013, 19: 3583-5395. 10.3748/wjg.v19.i23.3583.CrossRefPubMedPubMedCentral

25.

Kühnel K, Jarchau T, Wolf E, Schlichting I, Walter U, Wittinghofer A, Strelkov SV: The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat. Proc Natl Acad Sci U S A. 2004, 101: 17027-17032. 10.1073/pnas.0403069101.CrossRefPubMedPubMedCentral

26.

Comerford KM, Lawrence DW, Synnestvedt K, Levi BP, Colgan SP: Role of vasedi-Lator-stimulated phosphoprotein in PKA-induced changes in endothelial junctional permeability. FASEBJ. 2002, 16: 583-583.

27.

Luyer MD, Buurman WA, Hadfoune M, Jacobs JA, Konstantinov SR, Dejong CH, Greve JW: Pretreatment with high-fat enteral nutrition reduces endotoxin and tumor necrosis factor-alpha and preserves gut barrier function early after hemorrhagic shock. Shock. 2004, 21: 65-71.CrossRefPubMed

28.

Filos KS, Panteli ES, Fligou F, Papamichail C, Papapostolou I, Zervoudakis G, Spiliopoulou I, Georgiou C: Clonidine pre-treatment prevents hemorrhagic shock-induced endotoxemia and oxidative stress in the gut, liver, and lungs of the rat. Redox Rep. 2012, 17: 246-251. 10.1179/1351000212Y.0000000029.CrossRefPubMed

29.

Papia G, Fan J, Kapus A, Szaszi K, Marshall JC, Tawadros P, Ailenberg M, Rotstein OD: Altered inhibitory κBα expression in LPS-stimulated alveolar macrophages following resuscitated hemorrhagic shock. Shock. 2011, 35: 171-177. 10.1097/SHK.0b013e3181f21d2d.CrossRefPubMed

The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/80/prepub

Title: Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1
Authors: Xiaoguang Lu
Xin Kang
Libin Zhan
Chunyu Lv
Zhiwei Fan
Yingli Wang
Robbie Ali
Chang Lv
Siyao Li
Jinhai Mu
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-14-80

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2014

Berberine moderates glucose metabolism through the GnRH-GLP-1 and MAPK pathways in the intestine

An explorative study of metabolic responses to mental stress and yoga practices in yoga practitioners, non-yoga practitioners and individuals with metabolic syndrome

Gastrodia elata Blume alleviates L-DOPA-induced dyskinesia by normalizing FosB and ERK activation in a 6-OHDA-lesioned Parkinson’s disease mouse model

Changing use of traditional healthcare amongst those dying of HIV related disease and TB in rural South Africa from 2003 – 2011: a retrospective cohort study

A study of antioxidant activity, enzymatic inhibition and in vitro toxicity of selected traditional sudanese plants with anti-diabetic potential

A relaxation technique enhances psychological well-being and immune parameters in elderly people from a nursing home: A randomized controlled study