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BMC Complementary Medicine and Therapies

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Open Access 01-12-2009 | Research article

Cytotoxic and antibacterial activities of endophytic fungi isolated from plants at the National Park, Pahang, Malaysia

Authors: Nurul AMN Hazalin, Kalavathy Ramasamy, Siong Siong Meng Lim, Ibtisam Abdul Wahab, Anthony LJ Cole, Abu Bakar Abdul Majeed

Published in: BMC Complementary Medicine and Therapies | Issue 1/2009

Abstract

Background

Endophytes, microorganisms which reside in plant tissues, have potential in producing novel metabolites for exploitation in medicine. Cytotoxic and antibacterial activities of a total of 300 endophytic fungi were investigated.

Methods

Endophytic fungi were isolated from various parts of 43 plants from the National Park Pahang, Malaysia. Extracts from solid state culture were tested for cytotoxicity against a number of cancer cell lines using the MTT assay. Antibacterial activity was determined using the disc diffusion method.

Results

A total of 300 endophytes were isolated from various parts of plants from the National Park, Pahang. 3.3% of extracts showed potent (IC₅₀ < 0.01 μg/ml) cytotoxic activity against the murine leukemic P388 cell line and 1.7% against a human chronic myeloid leukemic cell line K562. Sporothrix sp. (KK29FL1) isolated from Costus speciosus showed strong cytotoxicity against colorectal carcinoma (HCT116) and human breast adenocarcinoma (MCF7) cell lines with IC₅₀ values of 0.05 μg/ml and 0.02 μg/ml, respectively. Antibacterial activity was demonstrated for 8% of the extracts.

Conclusion

Results indicate the potential for production of bioactive agents from endophytes of the tropical rainforest flora.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/9/46/prepub

Title: Cytotoxic and antibacterial activities of endophytic fungi isolated from plants at the National Park, Pahang, Malaysia
Authors: Nurul AMN Hazalin
Kalavathy Ramasamy
Siong Siong Meng Lim
Ibtisam Abdul Wahab
Anthony LJ Cole
Abu Bakar Abdul Majeed
Publication date: 01-12-2009
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2009
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-9-46

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cytotoxic and antibacterial activities of endophytic fungi isolated from plants at the National Park, Pahang, Malaysia

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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