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Open Access 01-03-2022 | Cytokines | Original Article

Intradialytic cycling does not exacerbate microparticles or circulating markers of systemic inflammation in haemodialysis patients

Authors: Patrick J. Highton, Daniel S. March, Darren R. Churchward, Charlotte E. Grantham, Hannah M. L. Young, Matthew P. M. Graham-Brown, Seila Estruel, Naomi Martin, Nigel J. Brunskill, Alice C. Smith, James O. Burton, Nicolette C. Bishop

Published in: European Journal of Applied Physiology | Issue 3/2022

Abstract

Purpose

Patients receiving haemodialysis (HD) display elevated circulating microparticle (MP) concentration, tissue factor (TF) expression and markers of systemic inflammation, though regular intradialytic cycling (IDC) may have a therapeutic effect. This study investigated the impact of regular, moderate-intensity IDC on circulating MPs and inflammatory markers in unit-based HD patients.

Methods

Patients were cluster-randomised to intervention (n = 20, age: 51.4 ± 18.1 years, body mass: 77.6 ± 18.3 kg, mean ± SD) or no-exercise control (n = 20, 56.8 ± 14.0 years, 80.5 ± 26.5 kg). Intervention participants completed 30 min of moderate intensity (rating of perceived exertion [RPE] of 12–14) IDC, thrice weekly for 6 months. Pre-dialysis venous blood samples were obtained at 0, 3 and 6 months. Circulating MP phenotypes, cytokines, chemokine and MP TF expression were quantified using flow cytometry and cytometric bead array assays.

Results

Despite high exercise compliance (82%), no IDC-dependent effects were observed for any MP, cytokine or chemokine measure (p ≥ 0.051, η_ρ² ≤ 0.399) other than TNF-α (p = 0.001, η_ρ² = 0.186), though no significance was revealed upon post hoc analysis.

Conclusion

Six months of regular, moderate-intensity IDC had no effect on MPs, cytokines or chemokines. This suggests that the exercise did not exacerbate thrombotic or inflammatory status, though further functional assays are required to confirm this.

Trial registration

ISRCTN1129707, prospectively registered on 05/03/2015.

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Title: Intradialytic cycling does not exacerbate microparticles or circulating markers of systemic inflammation in haemodialysis patients
Authors: Patrick J. Highton
Daniel S. March
Darren R. Churchward
Charlotte E. Grantham
Hannah M. L. Young
Matthew P. M. Graham-Brown
Seila Estruel
Naomi Martin
Nigel J. Brunskill
Alice C. Smith
James O. Burton
Nicolette C. Bishop
Publication date: 01-03-2022
Publisher: Springer Berlin Heidelberg
Keyword: Cytokines
Published in: European Journal of Applied Physiology / Issue 3/2022
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-021-04846-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Intradialytic cycling does not exacerbate microparticles or circulating markers of systemic inflammation in haemodialysis patients

Abstract

Purpose

Methods

Results

Conclusion

Trial registration

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

Trial registration

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