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Clinical Oral Investigations

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01-06-2021 | Cytokines | Original Article

Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats

Authors: Shuai Chen, Dianfu Ma, Suli Xiao, Pingping Li, Huaxiang Lei, Xiaojing Huang

Published in: Clinical Oral Investigations | Issue 6/2021

Abstract

Objectives

To study the effects of chronic apical periodontitis (CAP) on the inflammatory response and initial lesion of aorta in hyperlipemic rats.

Materials and methods

Sprague–Dawley (SD) rats aged 14 weeks were randomly divided into 4 experimental groups (n = 8), namely, normal diet (ND), high-fat diet (HFD), CAP, and HFD + CAP. The rats were raised under controlled conditions and fed with diet specified for each group. All subjects were euthanatized after 14 weeks for histopathological analysis. Serum cytokines were analyzed to assess changes in gene and protein expression of aorta via enzyme-linked immunosorbent assay and real-time polymerase chain reaction. Results were analyzed by one-way ANOVA.

Results

Low-density lipoprotein cholesterol levels in rats in HFD + CAP group were significantly higher than those in other groups. By comparison, low-density lipoprotein cholesterol levels in rats in both the HFD and HFD + CAP groups were significantly lower than those in the other groups. No significant difference among all groups was observed in terms of CRP level. However, levels of IL-2, IL-6, and IL-10 increased in the experimental CAP rats compared with the control rats. mRNA expression levels of MCP-1, TLR-4, and NF-κB p65 were markedly elevated in rats in the HFD group compared with those in rats in the ND group. TLR-4 mRNA expression was significantly higher in rats in the HFD + CAP group than that in rats in the HFD group.

Conclusions

CAP mediated the high expression of cytokines and induced the initial inflammatory response in the aorta. Apical periodontitis may affect the level of inflammatory cytokines (IL-2, IL-6, and IL-10) depending on the immune response.

Clinical relevance

CAP may trigger a systemic inflammatory response and affect the aorta of patients.

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Title: Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats
Authors: Shuai Chen
Dianfu Ma
Suli Xiao
Pingping Li
Huaxiang Lei
Xiaojing Huang
Publication date: 01-06-2021
Publisher: Springer Berlin Heidelberg
Keyword: Cytokines
Published in: Clinical Oral Investigations / Issue 6/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03714-6

At a glance: The STEP trials

Springer Medicine

Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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