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International Journal of Colorectal Disease

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01-03-2004 | Original Article

CXC and CC chemokine expression in inflamed and noninflamed pelvic ileal pouch tissue

Authors: Ulf Helwig, Paolo Gionchetti, Fernando Rizzello, Karen Lammers, Tanja Kühbacher, Stefan Schreiber, Marco Baggiolini, Mariagrazia Uguccioni, Massimo Campieri

Published in: International Journal of Colorectal Disease | Issue 2/2004

Abstract

Background and aims

Pouchitis is the major long-term complication after ileal pouch–anal anastomosis (IPAA) in patients operated on for ulcerative colitis. The cause is unknown, but both the history of ulcerative colitis and increased bacterial concentration are important factors. Chemokines are mediators for the recruitment of inflammatory cells to the site of inflammation. This study examined the tissue expression of a panel of specific chemokines and the corresponding recruitment of inflammatory cells in IPAA tissue with and without inflammation and after antibiotic treatment.

Patients and methods

Biopsy specimens postoperatively from ulcerative colitis patients with IPAA were obtained by endoscopy. Biopsies were taken from 8 patients with noninflamed IPAA and from 14 patients with an episode of acute pouchitis, before and after antibiotic treatment. Biopsies were stained for CD68, CD3, elastase, eotaxin, IP-10, MCP-1, MCP-3, and IL-8 and analyzed by NIH Image analyzer.

Results

Expression of IL-8, MCP-1, MCP-3, and IP-10 was significantly higher in pouchitis than normal pouch. The expression of MCP-1, MCP-3 and IP-10 were significantly lower after antibiotic treatment.

Conclusion

These data support the importance of chemokines for the leukocyte recruitment in pouch tissue during acute pouchitis.

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Title: CXC and CC chemokine expression in inflamed and noninflamed pelvic ileal pouch tissue
Authors: Ulf Helwig
Paolo Gionchetti
Fernando Rizzello
Karen Lammers
Tanja Kühbacher
Stefan Schreiber
Marco Baggiolini
Mariagrazia Uguccioni
Massimo Campieri
Publication date: 01-03-2004
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 2/2004
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-003-0500-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

CXC and CC chemokine expression in inflamed and noninflamed pelvic ileal pouch tissue

Abstract

Background and aims

Patients and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background and aims

Patients and methods

Results

Conclusion

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