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Tumor Biology
Published in: Tumor Biology 3/2016

01-03-2016 | Research Article

Curcumin induces apoptosis in p53-null Hep3B cells through a TAp73/DNp73-dependent pathway

Authors: Jinhong Wang, Hai Xie, Feng Gao, Tingkun Zhao, Hongming Yang, Bai Kang

Published in: Tumor Biology | Issue 3/2016

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Abstract

Curcumin has anticancer functions in various tumors. It has been shown to induce apoptosis through p53-dependent pathways. p73 gene is a member of the p53 family which encodes both a tumor suppressor (transactivation-competent p73 (TAp73)) and a putative oncogene (dominant-negative p73 (DNp73)); the former shares similarity with the tumor suppressor p53, and the latter behaves as dominant-negative proteins that interfere with the activity of TAp73. To understand the p73-dependent mechanisms that are engaged during curcumin-induced apoptosis, we established a p73 overexpression cell models using p53-deficient Hep3B cells (Hep3BTAp73/DNp73). Our results demonstrated that curcumin at concentrations of 40 and 80 μM induced DNA damage, increased TAp73/DNp73 ratio, and also led to apoptosis in the Hep3BTAp73/DNp73 cells. The apoptotic cell death was concurrent with the loss of mitochondrial membrane potential; release of cytochrome c from mitochondria; and the cleavage of caspase 9, caspase 3, and poly(ADP-ribose) polymerase (PARP). These results demonstrated a p73-dependent mechanism for curcumin-induced apoptosis that involves the mitochondria-mediated pathway.
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Metadata
Title
Curcumin induces apoptosis in p53-null Hep3B cells through a TAp73/DNp73-dependent pathway
Authors
Jinhong Wang
Hai Xie
Feng Gao
Tingkun Zhao
Hongming Yang
Bai Kang
Publication date
01-03-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 3/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-4029-3

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