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01-09-2010 | Experimental research

Curcumin improves early functional results after experimental spinal cord injury

Authors: Berker Cemil, Kivanc Topuz, Mehmet Nusret Demircan, Gokhan Kurt, Kagan Tun, Murat Kutlay, Osman Ipcioglu, Zafer Kucukodaci

Published in: Acta Neurochirurgica | Issue 9/2010

Abstract

Background

Curcumin is a polyphenol extracted from the rhizome of Curcuma longa and well known as a multifunctional drug with anti-oxidative, anticancerous, and anti-inflammatory activities. The aim of the study was to evaluate and compare the effects of the use of the curcumin and the methylprednisolone sodium succinate (MPSS) functionally, biochemically, and pathologically after experimental spinal cord injury (SCI).

Method

Forty rats were randomly allocated into five groups. Group 1 was performed only laminectomy. Group 2 was introduced 70-g closing force aneurysm clip injury. Group 3 was given 30 mg/kg MPSS intraperitoneally immediately after the trauma. Group 4 was given 200 mg/kg of curcumin immediately after the trauma. Group 5 was the vehicle, and immediately after trauma, 1 mL of rice bran oil was injected. The animals were examined by inclined plane score and Basso–Beattie–Bresnahan scale 24 h after the trauma. At the end of the experiment, spinal cord tissue samples were harvested to analyze tissue concentrations of malondialdehyde (MDA) levels, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity, and catalase (CAT) activity and pathological evaluation.

Findings

Curcumin treatment improved neurologic outcome, which was supported by decreased level of tissue MDA and increased levels of tissue GSH-Px, SOD, and CAT activity. Light microscopy results also showed preservation of tissue structure in the treatment group.

Conclusions

This study showed the neuroprotective effects of curcumin on experimental SCI model. By increasing tissue levels of GSH-Px, SOD, and CAT, curcumin seems to reduce the effects of injury to the spinal cord, which may be beneficial for neuronal survival.

Ammon HP, Wahl MA (1991) Pharmacology of Curcuma longa. Planta Med 57:1–7PubMedCrossRef

Anderson DK, Demediuk P, Saunders RD, Dugan LL, Means ED, Horrocks LA (1985) Spinal cord injury and protection. Ann Emerg Med 14:816–821PubMedCrossRef

Balentine JD (1978) Pathology of experimental spinal cord trauma. I. The necrotic lesion as a function of vascular injury. Lab Invest 39:236–253PubMed

Basso DM, Beattie MS, Bresnahan JC (1996) Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection. Exp Neurol 139:244–256PubMedCrossRef

Beril Gok H, Solaroglu I, Okutan O, Cimen B, Kaptanoglu E, Palaoglu S (2007) Metoprolol treatment decreases tissue myeloperoxidase activity after spinal cord injury in rats. J Clin Neurosci 14:138–142PubMedCrossRef

Bhutani MK, Bishnoi M, Kulkarni SK (2009) Anti-depressant like effect of curcumin and its combination with piperine in unpredictable chronic stress-induced behavioral, biochemical and neurochemical changes. Pharmacol Biochem Behav 92:39–43PubMedCrossRef

Bracken MB, Shepard MJ, Collins WF, Holford TR, Young W, Baskin DS, Eisenberg HM, Flamm E, Leo-Summers L, Maroon J (1990) A randomized, controlled trial of methylprednisolone or naloxone in the treatment of acute spinal-cord injury. Results of the Second National Acute Spinal Cord Injury Study. N Engl J Med 322:1405–1411PubMedCrossRef

Braughler JM, Hall ED (1983) Uptake and elimination of methylprednisolone from contused cat spinal cord following intravenous injection of the sodium succinate ester. J Neurosurg 58:538–542PubMedCrossRef

Cekmen M, Ilbey YO, Ozbek E, Simsek A, Somay A, Ersoz C (2009) Curcumin prevents oxidative renal damage induced by acetaminophen in rats. Food Chem Toxicol 47:1480–1484PubMedCrossRef

10.

Cheng AL, Hsu CH, Lin JK, Hsu MM, Ho YF, Shen TS, Ko JY, Lin JT, Lin BR, Ming-Shiang W, Yu HS, Jee SH, Chen GS, Chen TM, Chen CA, Lai MK, Pu YS, Pan MH, Wang YJ, Tsai CC, Hsieh CY (2001) Phase I clinical trial of curcumin, a chemopreventive agent, in patients with high-risk or pre-malignant lesions. Anticancer Res 21:2895–2900PubMed

11.

Church DF, Pryor WA (1985) Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 64:111–126PubMedCrossRef

12.

Dickens BF, Mak IT, Weglicki WB (1988) Lysosomal lipolytic enzymes, lipid peroxidation, and injury. Mol Cell Biochem 82:119–123PubMedCrossRef

13.

Dumont RJ, Okonkwo DO, Verma S, Hurlbert RJ, Boulos PT, Ellegala DB, Dumont AS (2001) Acute spinal cord injury, part I: pathophysiologic mechanisms. Clin Neuropharmacol 24:254–264PubMedCrossRef

14.

Ferrari R, Ceconi C, Curello S, Cargnoni A, Alfieri O, Pardini A, Marzollo P, Visioli O (1991) Oxygen free radicals and myocardial damage: protective role of thiol-containing agents. Am J Med 91:95–105CrossRef

15.

Ghoneim AI, Abdel-Naim AB, Khalifa AE, El-Denshary ES (2002) Protective effects of curcumin against ischaemia/reperfusion insult in rat forebrain. Pharmacol Res 46:273–279PubMedCrossRef

16.

Hall ED, Braughler JM (1989) Central nervous system trauma and stroke. II. Physiological and pharmacological evidence for involvement of oxygen radicals and lipid peroxidation. Free Radic Biol Med 6:303–313PubMedCrossRef

17.

Hall ED (1993) Lipid antioxidants in acute central nervous system injury. Ann Emerg Med 22:1022–1027PubMedCrossRef

18.

Halliwell B (1992) Reactive oxygen species and the central nervous system. J Neurochem 59:1609–1623PubMedCrossRef

19.

Jagetia GC (2007) Radioprotection and radiosensitization by curcumin. Adv Exp Med Biol 595:301–320PubMedCrossRef

20.

Joe B, Vijaykumar M, Lokesh BR (2004) Biological properties of curcumin-cellular and molecular mechanisms of action. Crit Rev Food Sci Nutr 44:97–111PubMedCrossRef

21.

Kahraman S, Düz B, Kayali H, Korkmaz A, Oter S, Aydin A, Sayal A (2007) Effects of methylprednisolone and hyperbaric oxygen on oxidative status after experimental spinal cord injury: a comparative study in rats. Neurochem Res 32:1547–1551PubMedCrossRef

22.

Kalayci M, Coskun O, Cagavi F, Kanter M, Armutcu F, Gul S, Acikgoz B (2005) Neuroprotective effects of ebselen on experimental spinal cord injury in rats. Neurochem Res 30:403–410PubMedCrossRef

23.

Kaptanoglu E, Caner HH, Sürücü HS, Akbiyik F (1999) Effect of mexiletine on lipid peroxidation and early ultrastructural findings in experimental spinal cord injury. J Neurosurg 91:200–204PubMed

24.

Kaptanoglu E, Okutan O, Akbiyik F, Solaroglu I, Kilinc A, Beskonakli E (2004) Correlation of injury severity and tissue Evans blue content, lipid peroxidation and clinical evaluation in acute spinal cord injury in rats. J Clin Neurosci 11:879–885PubMedCrossRef

25.

Kaptanoglu E, Tuncel M, Palaoglu S, Konan A, Demirpençe E, Kilinç K (2000) Comparison of the effects of melatonin and methylprednisolone in experimental spinal cord injury. J Neurosurg 93:77–84PubMedCrossRef

26.

King VR, Averill SA, Hewazy D, Priestley JV, Torup L, Michael-Titus AT (2007) Erythropoietin and carbamylated erythropoietin are neuroprotective following spinal cord hemisection in the rat. Eur J Neurosci 26:90–100PubMedCrossRef

27.

Kurt G, Ergün E, Cemil B, Börcek AO, Börcek P, Gülbahar O, Ceviker N (2009) Neuroprotective effects of infliximab in experimental spinal cord injury. Surg Neurol 71:332–336PubMedCrossRef

28.

Lin MS, Lee YH, Chiu WT, Hung KS (2010) Curcumin Provides Neuroprotection After Spinal Cord Injury. J Surg Res (in press)

29.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMed

30.

Mautes AE, Weinzierl MR, Donovan F, Noble LJ (2000) Vascular events after spinal cord injury: contribution to secondary pathogenesis. Phys Ther 80:673–687PubMed

31.

Motterlini R, Foresti R, Bassi R, Green CJ (2000) Curcumin, an antioxidant and anti-inflammatory agent, induces heme oxygenase-1 and protects endothelial cells against oxidative stress. Free Radic Biol Med 28:1303–1312PubMedCrossRef

32.

Nirmala C, Puvanakrishnan R (1996) Protective role of curcumin against isoproterenol induced myocardial infarction in rats. Mol Cell Biochem 159:85–93PubMedCrossRef

33.

Okada K, Wangpoengtrakul C, Tanaka T, Toyokuni S, Uchida K, Osawa T (2001) Curcumin and especially tetrahydrocurcumin ameliorate oxidative stress-induced renal injury in mice. J Nutr 131:2090–2095PubMed

34.

Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169PubMed

35.

Priyadarsini KI (1997) Free radical reactions of curcumin in membrane models. Free Radic Biol Med 23:838–843PubMedCrossRef

36.

Rivlin AS, Tator CH (1978) Effect of duration of acute spinal cord compression in a new acute cord injury model in the rat. Surg Neurol 10:38–43PubMed

37.

Sahin S, Söğüt S, Özyurt H, Uz E, İlhan A, Akyol Ö (2002) Tissue xanthine oxidase activity and nitric oxide levels after spinal cord ischemia/reperfusion injury in rabbits: comparison of caffeic acid phenethyl ester (CAPE) and methylprednisolone. Neurosci Res Commun 31:111–121CrossRef

38.

Saunders RD, Dugan LL, Demediuk P, Means ED, Horrocks LA, Anderson DK (1987) Effects of methylprednisolone and the combination of alpha-tocopherol and selenium on arachidonic acid metabolism and lipid peroxidation in traumatized spinal cord tissue. J Neurochem 49:24–31PubMedCrossRef

39.

Shen SQ, Zhang Y, Xiang JJ, Xiong CL (2007) Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes. World J Gastroenterol 13:1953–1961PubMed

40.

Shoskes D, Lapierre C, Cruz-Correa M, Muruve N, Rosario R, Fromkin B, Braun M, Copley J (2005) Beneficial effects of the bioflavonoids curcumin and quercetin on early function in cadaveric renal transplantation: a randomized placebo controlled trial. Transplantation 80:1556–1559PubMedCrossRef

41.

Shukla PK, Khanna VK, Khan MY, Srimal RC (2003) Protective effect of curcumin against lead neurotoxicity in rat. Hum Exp Toxicol 22:653–658PubMedCrossRef

42.

Simpson JI, Eide TR, Schiff GA, Clagnaz JF, Hossain I, Tverskoy A, Koski G (1994) Intrathecal magnesium sulfate protects the spinal cord from ischemic injury during thoracic aortic cross-clamping. Anesthesiology 81:1493–1499PubMedCrossRef

43.

Solaroglu I, Kaptanoglu E, Okutan O, Beskonakli E, Attar A, Kilinc K (2005) Magnesium sulfate treatment decreases caspase-3 activity after experimental spinal cord injury in rats. Surg Neurol 64:17–21CrossRef

44.

Sreejayan RMN (1994) Curcuminoids as potent inhibitors of lipid peroxidation. J Pharm Pharmacol 46:1013–1016PubMedCrossRef

45.

Sun Y (1990) Free radicals, antioxidant enzymes, and carcinogenesis. Free Radic Biol Med 8:583–599PubMedCrossRef

46.

Tator CH (1998) Biology of neurological recovery and functional restoration after spinal cord injury. Neurosurgery 42:696–707PubMedCrossRef

47.

Wang Q, Sun AY, Simonyi A, Jensen MD, Shelat PB, Rottinghaus GE, MacDonald RS, Miller DK, Lubahn DE, Weisman GA, Sun GY (2005) Neuroprotective mechanisms of curcumin against cerebral ischemia-induced neuronal apoptosis and behavioral deficits. J Neurosci Res 82:138–148PubMedCrossRef

48.

Yang YB, Piao YJ (2003) Effects of resveratrol on secondary damages after acute spinal cord injury in rats. Acta Pharmacol Sin 24:703–710PubMed

49.

Zbarsky V, Datla KP, Parkar S, Rai DK, Aruoma OI, Dexter DT (2005) Neuroprotective properties of the natural phenolic antioxidants curcumin and naringenin but not quercetin and fisetin in a 6-OHDA model of Parkinson's disease. Free Radic Res 39:1119–1125PubMedCrossRef

50.

Zhao J, Yu S, Zheng W, Feng G, Luo G, Wang L, Zhao Y (2009) Curcumin improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats. Neurochem Res 35:374–379PubMedCrossRef

Title: Curcumin improves early functional results after experimental spinal cord injury
Authors: Berker Cemil
Kivanc Topuz
Mehmet Nusret Demircan
Gokhan Kurt
Kagan Tun
Murat Kutlay
Osman Ipcioglu
Zafer Kucukodaci
Publication date: 01-09-2010
Publisher: Springer Vienna
Published in: Acta Neurochirurgica / Issue 9/2010
Print ISSN: 0001-6268
Electronic ISSN: 0942-0940
DOI: https://doi.org/10.1007/s00701-010-0702-x

2024 ASCO Annual Meeting

Springer Medicine

Curcumin improves early functional results after experimental spinal cord injury

Abstract

Background

Method

Findings

Conclusions

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Background

Method

Findings

Conclusions

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