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Fluids and Barriers of the CNS
Published in: Fluids and Barriers of the CNS 1/2009

Open Access 01-12-2009 | Research

CSF/serum quotient graphs for the evaluation of intrathecal C4synthesis

Authors: Barbara Padilla-Docal, Alberto J Dorta-Contreras, Raisa Bu-Coifiu-Fanego, Alexis Rodriguez Rey

Published in: Fluids and Barriers of the CNS | Issue 1/2009

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Abstract

Background

Cerebrospinal fluid (CSF)/serum quotient graphs have been used previously to determine local synthesis in brain of immunoglobulins and C3 complement component. The aim of this study was to use the same technique to construct quotient graphs, or Reibergrams, for the beta globulin C4 and to evaluate the method for assessing intrathecal synthesis in neurological disease.

Methods

The constants in the previously-defined Reibergram for immunoglobulin IgA were used to calculate the CSF/serum quotient for C4. CSF and serum were analyzed for C4, IgA and albumin from a total of 12 patients with meningoencephalitis caused by encapsulated microorganisms and 10 subjects without infections or inflammatory neurological disease, some of which had dysfunction of the blood-CSF barrier,

Results

The formula and C4 Reibergram with the constants previously found for IgA, determined the intrathecal C4 synthesis in CSF. The intrathecal C4 fraction in CSF (C4 loc in mg/l) was compared to the C4-Index (fraction of CSF: serum for C 4/fraction of CSF: serum for albumin). There was a significant correlation between the two formulae. The CSF/Serum quotient graph was superior for detecting intrathecal synthesis of C4 under variable conditions of blood-CSF barrier permeability.

Conclusion

The C4 Reibergram can be used to quantify the intrathecal synthesis of this component of the complement system in different infectious diseases of the central nervous system and is especially useful for patients with blood-brain barrier dysfunction.
Appendix
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Metadata
Title
CSF/serum quotient graphs for the evaluation of intrathecal C4synthesis
Authors
Barbara Padilla-Docal
Alberto J Dorta-Contreras
Raisa Bu-Coifiu-Fanego
Alexis Rodriguez Rey
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Fluids and Barriers of the CNS / Issue 1/2009
Electronic ISSN: 2045-8118
DOI
https://doi.org/10.1186/1743-8454-6-8

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