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EJNMMI Research
Published in: EJNMMI Research 1/2017

Open Access 01-12-2017 | Letter to Editor

Cryogenic transmission electron microscopy: the technique of choice for the characterization of polymeric nanocarriers

Authors: Adrianus C. Laan, Antonia G. Denkova

Published in: EJNMMI Research | Issue 1/2017

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Abstract

This letter is meant to make scientists aware of the proper application of transmission electron microscopy (TEM) for the assessment of polymeric self-assemblies. Cryogenic (cryo)-TEM should be the method of choice. Here, we show the difference in morphologies observed in the same sample when using cryo-TEM and when using TEM with drying, demonstrating the importance of choosing the proper method.
Literature
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Laan AC, Santini C, Jennings L, de Jong M, Bernsen MR, Denkova AG. Radiolabeling polymeric micelles for in vivo evaluation: a novel, fast, and facile method. EJNMMI Res. 2016;6:12.CrossRefPubMedPubMedCentral
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Franken LE, Boekema EJ, Stuart MCA: Transmission electron microscopy as a tool for the characterization of soft materials: application and interpretation. Adv Sci. 2017: 1600476
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Patterson JP, Robin MP, Chassenieux C, Colombanib O, O’Reilly RK. The analysis of solution self-assembled polymeric nanomaterials. Chem Soc Rev. 2014;43:2412–25.CrossRefPubMed
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Friedrich H, Frederik PM, de With G, Sommerdijk NAJM. Imaging of self-assembled structures: interpretation of TEM and cryo-TEM images. Angew Chem Int Ed. 2010;49:7850–8.CrossRef
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Cui H, Hodgdon TK, Kaler EW, Abezgauz L, Danino D, Lubovsky M, Talmond Y, Pochan DJ. Elucidating the assembled structure of amphiphiles in solution via cryogenic transmission electron microscopy. Soft Matter. 2007;3:945–55.CrossRef
Metadata
Title
Cryogenic transmission electron microscopy: the technique of choice for the characterization of polymeric nanocarriers
Authors
Adrianus C. Laan
Antonia G. Denkova
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
EJNMMI Research / Issue 1/2017
Electronic ISSN: 2191-219X
DOI
https://doi.org/10.1186/s13550-017-0290-3

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