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Neuroscience Bulletin
Published in: Neuroscience Bulletin 4/2019

01-08-2019 | Original Article

Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono- and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers

Authors: Pengjie Wen, Xiaoping Rao, Liuying Xu, Zhijian Zhang, Fan Jia, Xiaobin He, Fuqiang Xu

Published in: Neuroscience Bulletin | Issue 4/2019

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Abstract

Sensory processing is strongly modulated by different brain and behavioral states, and this is based on the top–down modulation. In the olfactory system, local neural circuits in the olfactory bulb (OB) are innervated by centrifugal afferents in order to regulate the processing of olfactory information in the OB under different behavioral states. The purpose of the present study was to explore the organization of neural networks in olfactory-related cortices and modulatory nuclei that give rise to direct and indirect innervations to the glomerular layer (GL) of the OB at the whole-brain scale. Injection of different recombinant attenuated neurotropic viruses into the GL showed that it received direct inputs from each layer in the OB, centrifugal inputs from the ipsilateralanterior olfactory nucleus (AON), anterior piriform cortex (Pir), and horizontal limb of diagonal band of Broca (HDB), and various indirect inputs from bilateral cortical neurons in the AON, Pir, amygdala, entorhinal cortex, hippocampus, HDB, dorsal raphe, median raphe and locus coeruleus. These results provide a circuitry basis that will help further understand the mechanism by which olfactory information-processing in the OB is regulated.
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Metadata
Title
Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono- and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers
Authors
Pengjie Wen
Xiaoping Rao
Liuying Xu
Zhijian Zhang
Fan Jia
Xiaobin He
Fuqiang Xu
Publication date
01-08-2019
Publisher
Springer Singapore
Published in
Neuroscience Bulletin / Issue 4/2019
Print ISSN: 1673-7067
Electronic ISSN: 1995-8218
DOI
https://doi.org/10.1007/s12264-019-00385-6

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