Fig. 9
Effects of PKC activators and inhibitors on the quercetin induced decrease in expression of PKC-α. B16-BL6 cells were incubated with quercetin alone or PMA (100 nM), FFA (6.7 mM), doxorubicin (8.6 μM) and chlorpromazine (14 mM) in the presence or absence of quercetin for 24 h. a The levels of expression of PKC-α, PKC-β, and PKC-δ were determined by Western blotting (lane 1 control, lane 2 quercetin alone, lane 3 PMA, lane 4 quercetin + PMA, lane 5 FFA, lane 6 quercetin + FFA, lane 7 doxorubicin, lane 8 quercetin + doxorubicin, lane 9 chlorpromazine, lane 10 quercetin + chlorpromazine. b The relative expression of PKC-α, PKC-β, and PKC-δ in B16-BL6 cells was quantified densitometrically using the software Image J and calculated according to the reference bands of the control. The data presented are representative of three independent experiments