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01-06-2011 | Original Article

Continuous Monitoring of Changes in Plasma Nitrite Following Cerebral Ischemia in a Rabbit Embolic Stroke Model

Authors: James H. Silver, Paul A. Lapchak

Published in: Translational Stroke Research | Issue 2/2011

Abstract

In this proof-of-concept study, we investigated direct, continuous monitoring of plasma nitrite as an indicator of cerebral ischemia following clot embolization of rabbits via an indwelling carotid catheter. Two groups of rabbits were studied to compare the effects of embolization on nitrite levels. In the control group, blood was continuously obtained from a jugular venous catheter. The blood was immediately passed through an ultrafiltration filter; the filtrate was chemically reduced to convert free nitrite to nitric oxide (NO) and then measured using a NO-specific electrode. In the embolized group, after a baseline nitrite level was achieved, blood clots were injected into the brain via the carotid artery catheter, and then nitrite levels were continuously measured from jugular venous blood. The stroke group showed a significantly greater increase in nitrite as compared to controls (p = 0.017). Using the area-under-the-curve (AUC) method, results reached statistical significance (p < 0.05) within 3 min of embolization. In embolized rabbits, NO₂ levels increased 424 ± 256% compared to baseline. This study shows that nitrite can be measured immediately following a stroke and that our system measures nitrite independent of the extent of the stroke. This study provides evidence for the feasibility of using nitrite as a marker of ischemic stroke.

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Title: Continuous Monitoring of Changes in Plasma Nitrite Following Cerebral Ischemia in a Rabbit Embolic Stroke Model
Authors: James H. Silver
Paul A. Lapchak
Publication date: 01-06-2011
Publisher: Springer-Verlag
Published in: Translational Stroke Research / Issue 2/2011
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI: https://doi.org/10.1007/s12975-011-0073-5

At a glance: The STEP trials

Springer Medicine

Continuous Monitoring of Changes in Plasma Nitrite Following Cerebral Ischemia in a Rabbit Embolic Stroke Model

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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