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Clinical Orthopaedics and Related Research®
Published in: Clinical Orthopaedics and Related Research® 1/2011

01-01-2011 | Symposium: Papers Presented at the Annual Meetings of the Knee Society

Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis

Authors: Pei-Gen Ren, DVM, PhD, Afraaz Irani, BS, Zhinong Huang, MD, PhD, Ting Ma, MD, MSc, Sandip Biswal, MD, Stuart B. Goodman, MD, PhD

Published in: Clinical Orthopaedics and Related Research® | Issue 1/2011

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Abstract

Background

Aseptic loosening and periprosthetic osteolysis resulting from wear debris are major complications of total joint arthroplasty. Monocyte/macrophages are the key cells related to osteolysis at the bone-implant interface of joint arthroplasties. Whether the monocyte/macrophages found at the implant interface in the presence of polyethylene particles are locally or systemically derived is unknown.

Questions/purposes

We therefore asked (1) whether macrophages associated with polyethylene particle-induced chronic inflammation are recruited locally or systemically and (2) whether the recruited macrophages are associated with enhanced osteolysis locally.

Methods

Noninvasive in vivo imaging techniques (bioluminescence and microCT) were used to investigate initial macrophage migration systemically from a remote injection site to polyethylene wear particles continuously infused into the femoral canal. We used histologic and immunohistologic staining to confirm localization of migrated macrophages to the polyethylene particle-treated femoral canals and monitor cellular markers of bone remodeling.

Results

The values for bioluminescence were increased for animals receiving UHMWPE particles compared with the group in which the carrier saline was infused. At Day 8, the ratio of bioluminescence (operated femur divided by nonoperated contralateral femur of each animal) for the UHMWPE group was 13.95 ± 5.65, whereas the ratio for the saline group was 2.60 ± 1.14. Immunohistologic analysis demonstrated the presence of reporter macrophages in the UHMWPE particle-implanted femora only. MicroCT scans showed the bone mineral density for the group with both UHMWPE particles and macrophage was lower than the control groups.

Conclusions

Infusion of clinically relevant polyethylene particles, similar to the human scenario, stimulated systemic migration of remotely injected macrophages and local net bone resorption.
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Metadata
Title
Continuous Infusion of UHMWPE Particles Induces Increased Bone Macrophages and Osteolysis
Authors
Pei-Gen Ren, DVM, PhD
Afraaz Irani, BS
Zhinong Huang, MD, PhD
Ting Ma, MD, MSc
Sandip Biswal, MD
Stuart B. Goodman, MD, PhD
Publication date
01-01-2011
Publisher
Springer-Verlag
Published in
Clinical Orthopaedics and Related Research® / Issue 1/2011
Print ISSN: 0009-921X
Electronic ISSN: 1528-1132
DOI
https://doi.org/10.1007/s11999-010-1645-5

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