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European Journal of Medical Research

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Open Access 01-12-2014 | Research

Construction of microRNA and transcription factor regulatory network based on gene expression data in cardiomyopathy

Authors: Lei Wang, Jihua Hu, Haijian Xing, Min Sun, Juanli Wang, Qiang Jian, Hua Yang

Published in: European Journal of Medical Research | Issue 1/2014

Abstract

Background

Cardiomyopathy is a progressive myocardial disorder. Here, we attempted to reveal the possible mechanism of cardiomyopathy at the transcription level with the roles of microRNAs (miRNAs) and transcription factors (TFs) taken into account.

Method

We firstly identified differentially expressed genes (DEGs) between cardiomyopathy patients and controls with data from the gene expression omnibus (GEO) database. DEGs were associated with the canonical pathways, molecular and cellular functions, physiological system development and function in the Ingenuity Knowledge Base by using the Ingenuity Pathway Analysis (IPA) software. TFs and miRNAs that DEGs significantly enriched were identified and a double-factor regulatory network was constructed.

Results

A total of 1,680 DEGs were identified. The DEGs were enriched for various pathways, with glucocorticoid receptor signaling as the most significant. A double-factor regulatory network was constructed, including seven TFs and two miRNAs. A subnetwork under the regulation of MEF2C and SRF was also constructed to illustrate their regulatory effects on cardiac functions.

Conclusion

Our results may provide new understanding of cardiomyopathy and may facilitate further therapeutic studies.

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Title: Construction of microRNA and transcription factor regulatory network based on gene expression data in cardiomyopathy
Authors: Lei Wang
Jihua Hu
Haijian Xing
Min Sun
Juanli Wang
Qiang Jian
Hua Yang
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2014
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/s40001-014-0057-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Construction of microRNA and transcription factor regulatory network based on gene expression data in cardiomyopathy

Abstract

Background

Method

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Method

Results

Conclusion

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