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Virology Journal
Published in: Virology Journal 1/2007

Open Access 01-12-2007 | Short report

Conserved aspartic acid 233 and alanine 231 are not required for poliovirus polymerase function in replicons

Authors: Marion S Freistadt, Karen E Eberle

Published in: Virology Journal | Issue 1/2007

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Abstract

Nucleic acid polymerases have similar structures and motifs. The function of an aspartic acid (conserved in all classes of nucleic acid polymerases) in motif A remains poorly understood in RNA-dependent RNA polymerases. We mutated this residue to alanine in a poliovirus replicon. The resulting mutant could still replicate, although at a reduced level. In addition, mutation A231C (also in motif A) yielded high levels of replication. Taken together these results show that poliovirus polymerase conserved residues D233 and A231 are not essential to poliovirus replicon function.
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Metadata
Title
Conserved aspartic acid 233 and alanine 231 are not required for poliovirus polymerase function in replicons
Authors
Marion S Freistadt
Karen E Eberle
Publication date
01-12-2007
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2007
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-4-28

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