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01-09-2006 | Original Article

Conjugated linoleic acid reduced metastasized LL2 tumors in mouse peritoneum

Authors: Yasuha Sakai, Tomonori Sasahira, Hitoshi Ohmori, Kazuhiro Yoshida, Hiroki Kuniyasu

Published in: Virchows Archiv | Issue 3/2006

Abstract

The effect of conjugated linoleic acid (CLA) on pre-existent peritoneal metastasis was examined by mouse peritoneal metastasis models. The cell growth of LL2 mouse cancer cells was suppressed by CLA in a dose-dependent manner. CLA-induced growth inhibition was recovered by the exposure to antisense S-oligodeoxynucleotide for peroxisome proliferator-activated receptor (PPAR)-γ. C57B6 mice were inoculated with LL2 cells into their peritoneal cavity. Two weeks after inoculation, colonized peritoneal cancer foci (2.2±0.4 mm in diameter) were treated with CLA administrated intraperitoneally (200 or 600 pmol/mouse, twice a week). CLA treatment decreased the number of peritoneal tumors: 8.7±0.6, 5.7±0.6, and 2.3±0.6 in untreated, 200 pmol/mouse CLA, and 600 pmol/mouse CLA groups, respectively (P<0.0001). CLA treatment decreased the size of peritoneal tumors: 3.7±1.5, 1.3±0.5, and 1.0±0.4 mm in untreated, 200 pmol/mouse CLA, and 600 pmol/mouse CLA groups, respectively (P<0.0001). In CLA-treated tumors, proliferating cells were decreased (P<0.0001), whereas apoptotic cells were increased (P=0.0010). CLA-treated LL2 tumors showed decrease of PPARγ and EGFR proteins and increase of BAX protein in comparison with untreated tumors. These findings suggest that CLA possesses anti-tumor capability to peritoneal metastasis.

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Title: Conjugated linoleic acid reduced metastasized LL2 tumors in mouse peritoneum
Authors: Yasuha Sakai
Tomonori Sasahira
Hitoshi Ohmori
Kazuhiro Yoshida
Hiroki Kuniyasu
Publication date: 01-09-2006
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 3/2006
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-006-0249-7

At a glance: The STEP trials

Springer Medicine

Conjugated linoleic acid reduced metastasized LL2 tumors in mouse peritoneum

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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