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01-12-2017 | Original Article

Concomitant external pneumatic compression treatment with consecutive days of high intensity interval training reduces markers of proteolysis

Authors: Cody T. Haun, Michael D. Roberts, Matthew A. Romero, Shelby C. Osburn, James C. Healy, Angelique N. Moore, Christopher B. Mobley, Paul A. Roberson, Wesley C. Kephart, Petey W. Mumford, Michael D. Goodlett, David D. Pascoe, Jeffrey S. Martin

Published in: European Journal of Applied Physiology | Issue 12/2017

Abstract

Purpose

To compare the effects of external pneumatic compression (EPC) and sham when used concurrently with high intensity interval training (HIIT) on performance-related outcomes and recovery-related molecular measures.

Methods

Eighteen recreationally endurance-trained male participants (age: 21.6 ± 2.4 years, BMI: 25.7 ± 0.5 kg/m², VO_2peak: 51.3 ± 0.9 mL/kg/min) were randomized to balanced sham and EPC treatment groups. Three consecutive days of HIIT followed by EPC/sham treatment (Days 2–4) and 3 consecutive days of recovery (Days 5–7) with EPC/sham only on Days 5–6 were employed. Venipuncture, flexibility and pressure-to-pain threshold (PPT) measurements were made throughout. Vastus lateralis muscle was biopsied at PRE (i.e., Day 1), 1-h post-EPC/sham treatment on Day 2 (POST1), and 24-h post-EPC/sham treatment on Day 7 (POST2). 6-km run time trial performance was tested at PRE and POST2.

Results

No group × time interaction was observed for flexibility, PPT, or serum measures of creatine kinase (CK), hsCRP, and 8-isoprostane. However, there was a main effect of time for serum CK (p = 0.005). Change from PRE in 6-km run times at POST2 were not significantly different between groups. Significant between-groups differences existed for change from PRE in atrogin-1 mRNA (p = 0.018) at the POST1 time point (EPC: − 19.7 ± 8.1%, sham: + 7.7 ± 5.9%) and atrogin-1 protein concentration (p = 0.013) at the POST2 time point (EPC: − 31.8 ± 7.5%, sham: + 96.0 ± 34.7%). In addition, change from PRE in poly-Ub proteins was significantly different between groups at both the POST1 (EPC: − 26.0 ± 10.3%, sham: + 34.8 ± 28.5%; p = 0.046) and POST2 (EPC: − 33.7 ± 17.2%, sham: + 21.4 ± 14.9%; p = 0.037) time points.

Conclusions

EPC when used concurrently with HIIT and in subsequent recovery days reduces skeletal muscle markers of proteolysis.

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Title: Concomitant external pneumatic compression treatment with consecutive days of high intensity interval training reduces markers of proteolysis
Authors: Cody T. Haun
Michael D. Roberts
Matthew A. Romero
Shelby C. Osburn
James C. Healy
Angelique N. Moore
Christopher B. Mobley
Paul A. Roberson
Wesley C. Kephart
Petey W. Mumford
Michael D. Goodlett
David D. Pascoe
Jeffrey S. Martin
Publication date: 01-12-2017
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 12/2017
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-017-3746-2

At a glance: The STEP trials

Springer Medicine

Concomitant external pneumatic compression treatment with consecutive days of high intensity interval training reduces markers of proteolysis

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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