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01-02-2013 | Cardiac

Comprehensive phenotyping of salt-induced hypertensive heart disease in living mice using cardiac magnetic resonance

Authors: Hubert Cochet, William Lefrançois, Michel Montaudon, François Laurent, Line Pourtau, Sylvain Miraux, Elodie Parzy, Jean-Michel Franconi, Eric Thiaudière

Published in: European Radiology | Issue 2/2013

Abstract

Objectives

To characterise the effects of high-salt diet (HSD) on left ventricular (LV) mass, systolic function and coronary reserve in living mice using cardiac magnetic resonance imaging (MRI).

Methods

Thirty C57BL/6 1-month-old female mice were fed either a control (n = 15) or an HSD (n = 15). After 3 months, LV volumes, ejection fraction and mass were assessed using time-resolved three-dimensional (3D) black-blood manganese-enhanced MRI, and coronary flow velocity reserve (CFVR) was assessed using dynamic MR angiography at rest and during adenosine-induced hyperaemia. Hearts were excised to assess LV wet mass and micro-vascular remodelling at histology.

Results

Micro-vascular remodelling was found at histology in all investigated hearts from the HSD group and none from the control group. No difference between the HSD and control groups was found in terms of heart weight, LV volumes and ejection fraction. Heart to body weight ratio was higher in the HSD group (4.39 ± 0.24 vs 4.02 ± 0.16 mg/g, P < 0.001), because of lower body weight (22.3 ± 0.9 vs 24.0 ± 1.4 g, P < 0.001). CFVR was lower in the HSD group (1.73 ± 0.11 vs 1.94 ± 0.12, P < 0.001).

Conclusions

Phenotyping of hypertensive heart disease is feasible in living mice using dynamic MR angiography and time-resolved 3D black-blood manganese-enhanced MRI. HSD is associated with early impairment of coronary reserve, before the onset of significant hypertrophy.

Key Points

• In vivo phenotyping of hypertensive heart disease is feasible in mice using MRI

• HSD in mice is associated with early impairment of coronary reserve

• Dietary salt in mice alters coronary reserve before the onset of ventricular hypertrophy

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Title: Comprehensive phenotyping of salt-induced hypertensive heart disease in living mice using cardiac magnetic resonance
Authors: Hubert Cochet
William Lefrançois
Michel Montaudon
François Laurent
Line Pourtau
Sylvain Miraux
Elodie Parzy
Jean-Michel Franconi
Eric Thiaudière
Publication date: 01-02-2013
Publisher: Springer-Verlag
Published in: European Radiology / Issue 2/2013
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-012-2598-8

At a glance: The STEP trials

Springer Medicine

Comprehensive phenotyping of salt-induced hypertensive heart disease in living mice using cardiac magnetic resonance

Abstract

Objectives

Methods

Results

Conclusions

Key Points

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Methods

Results

Conclusions

Key Points

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