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01-12-2017 | Brief Report

Comprehensive characterization of a major capsid protein derived from a documented GII.6 norovirus strain

Authors: Lijun Zheng, Wenhui Wang, Jinjin Liu, Yuqi Huo, Chuan Qin, Mingchen Wang, Shuo Shen

Published in: Archives of Virology | Issue 12/2017

Abstract

In this study, we successfully produced VLPs derived from full-length or chimeric VP1 of a documented GII.6 strain. Trypsin digestion of purified VLPs led to total cleavage of VP1, while the integrity of assembled VLPs was not affected. In vitro VLP-histo-blood group antigen (HBGA) binding and binding blockade assays indicated that trypsin digestion enhanced the binding of GII.6 VLPs to salivary HBGAs and that this binding could only be blocked by serum produced against a homologous strain. The data regarding the assembly, morphology and binding patterns of GII.6 NoV VLPs presented here might be useful for further study of GII.6 NoVs.

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Title: Comprehensive characterization of a major capsid protein derived from a documented GII.6 norovirus strain
Authors: Lijun Zheng
Wenhui Wang
Jinjin Liu
Yuqi Huo
Chuan Qin
Mingchen Wang
Shuo Shen
Publication date: 01-12-2017
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 12/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3537-4

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