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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-11-2007 | Short Communication

Complement factor H variant Y402H and basal laminar deposits in exudative age-related macular degeneration

Authors: Albrecht Lommatzsch, Pia Hermans, Bernhard Weber, Daniel Pauleikhoff

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 11/2007

Abstract

Background

Complement factor H (CFH) polymorphism Y402H has been shown to be significantly associated with age-related macular degeneration (AMD). Furthermore, histopathological studies in AMD have implicated basal laminar deposits (BLD) in the development of choroidal neovascularization (CNV) membranes. The purpose of this study was to correlate CFH staining in BLD with the CFH genotype at the tyrosine 402 histidine (Y402H) polymorphism.

Patients and methods

During macular translocation, 21 angiographically confirmed CNV membranes were extracted in 21 patients. The specimens were analysed histologically for BLD. The presence of CFH, complement proteins, and vitronectin was determined by immunohistochemistry. Finally, the CFH Y402H genotype was established by direct sequencing analysis.

Results

Histological examination demonstrated BLD in all of the excised CNV membranes. By immunostaining CFH was detected in the peripheral aspect at the inner and outer surface of BLD, which colocalized with other proteins of the complement cascade (C3, C5b-9). Similarly, vitronectin was detected in all of the BLD investigated. Four patients were noncarriers of CFH Y402H polymorphism, nine patients were heterozygous and eight patients homozygous for the CFH Y402H polymorphism.

Conclusions

BLD are composed of different complement factors (factor H, C3, C5b-9) and extracellular matrix proteins such as vitronectin. The prevalence of homozygous carriers in regard to CFH Y402H polymorphism, which is suspicious for AMD, might be associated with increased secretion of vitronectin in response to dysregulation of the complement cascade.

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Title: Complement factor H variant Y402H and basal laminar deposits in exudative age-related macular degeneration
Authors: Albrecht Lommatzsch
Pia Hermans
Bernhard Weber
Daniel Pauleikhoff
Publication date: 01-11-2007
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 11/2007
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-007-0649-7

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Springer Medicine

Complement factor H variant Y402H and basal laminar deposits in exudative age-related macular degeneration

Abstract

Background

Patients and methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Patients and methods

Results

Conclusions

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