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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2010

Open Access 01-12-2010 | Research

Comparison of the multi-drug resistant human hepatocellular carcinoma cell line Bel-7402/ADM model established by three methods

Authors: Xingguo Zhong, Maoming Xiong, Xiangling Meng, Renhua Gong

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2010

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Abstract

Background

To compare the biological characteristics of three types of human hepatocellular carcinoma multi-drug resistant cell sub-lines Bel-7402/ADM models established by three methods.

Methods

Established human hepatocellular carcinoma adriamycin (ADM) multi-drug resistant cell sub-lines models Bel-7402/ADMV, Bel-7402/ADML and Bel-7402/ADMS by three methods of in vitro concentration gradient increased induction, nude mice liver-implanted induction and subcutaneous-implanted induction respectively. Phase contrast microscopy was used to observe the cells and the MTT (methyl thiazolyl tetrazolium) method was used to detect drug resistance of the three different sub-lines of cells.

Results

The three groups of drug resistant cells, Bel-7402/ADMV, Bel-7402/ADML and Bel-7402/ADMS generated cross-resistance to ADM and CDDP (cis-Diaminedichloroplatinum), but showed a significant difference in resistance to Bel-7402 IC50 value (P < 0.01). The doubling times were significantly extended compared to the parent cell line (39 h) and were 65 h (Bel-7402/ADMV), 46 h (Bel-7402/ADML), and 45 h (Bel-7402/ADMS). The excretion rates of ADM were significantly increased compared with the parent cell (34.14%) line and were 81.06% (Bel-7402/ADMV), 66.56% (Bel-7402/ADML) and 61.56% (Bel-7402/ADMS). Expression of P-gp and MRP in the three groups of resistant cells was significantly enhanced (P < 0.01). There was no significant variation in the expression of GSH/GST (P > 0.05).

Conclusions

Stable resistance was involved in the resistant cell line model established by the above three methods. Liver implantation was a good simulation of human hepatocellular and proved to be an ideal model with characteristics similar to human hepatocellular biology and the pharmacokinetics of anticancer drugs.
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Literature
1.
Kessel D, Botterill V, Wodinsky I: Uptake and retention of daunomycin by mouse leukemic cells as factors in drug response. Cancer Res. 1968, 28: 938-941.
2.
Biedler JL, Riehm H: Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies. Cancer Res. 1970, 30: 1174-1184.
3.
Zhou XD, Tang ZY, Yang BH, Lin ZY, Ma ZC, Ye SL, Wu ZQ, Fan J, Qin LX, Zheng BH: Experience of 1000 patients who underwent hepatectomy for small hepatocellular carcinoma. Cancer. 2001, 91: 1479-1486. 10.1002/1097-0142(20010415)91:8<1479::AID-CNCR1155>3.0.CO;2-0.CrossRef
4.
Yang JM, Kan T, Chen H, Wu MC: Hepatectomy in the treatment of very big primary liver cancer: report of 86 cases. Hepatobiliary Pancreat Dis Int. 2002, 1: 42-45.
5.
Pignata S, Daniele B, Gallo C, De Vivo R, Monfardini S, Perrone F: Endocrine treatment of hepatocellular carcinoma. Any evidence of benefit?. Eur J Cancer. 1998, 34: 25-32. 10.1016/S0959-8049(97)00317-1.CrossRef
6.
Urasaki Y, Ueda T, Yoshida A, Fukushima T, Takeuchi N, Tsuruo T, Nakamura T: Establishment of a daunorubicin-resistant cell line which shows multi-drug resistance by multifactorial mechanisms. Anticancer Res. 1996, 16: 709-714.
7.
Yang LY, Trujillo JM: Biological characterization of multidrug-resistant human colon carcinoma sublines induced/selected by two methods. Cancer Res. 1990, 50: 3218-3225.
8.
Gottesman MM, Fojo T, Bates SE: Multidrug resistance in cancer: role of ATP-dependent transporters. Nat Rev Cancer. 2002, 2: 48-58. 10.1038/nrc706.CrossRef
9.
Juliano RL, Ling V: A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim Biophys Acta. 1976, 455: 152-162. 10.1016/0005-2736(76)90160-7.CrossRef
10.
Endo K, Maehara Y, Ichiyoshi Y, Kusumoto T, Sakaguchi Y, Ohno S, Sugimachi K: Multidrug resistance-associated protein expression in clinical gastric carcinoma. Cancer. 1996, 77: 1681-1687.CrossRef
11.
Correnti M, Cavazza ME, Guedez N, Herrera O, Suarez-Chacon NR: Expression of the multidrug-resistance (MDR) gene in breast cancer. J Chemother. 1995, 7: 449-451.CrossRef
12.
Rafki N, Liautaud-Roger F, Devy L, Trentesaux C, Dufer J: P53 protein expression in human multidrug-resistant CEM lymphoblasts. Leuk Res. 1997, 21: 147-152. 10.1016/S0145-2126(96)00086-0.CrossRef
13.
Siegel DS, Zhang X, Feinman R, Teitz T, Zelenetz A, Richon VM, Rifkind RA, Marks PA, Michaeli J: Hexamethylene bisacetamide induces programmed cell death (apoptosis) and down-regulates BCL-2 expression in human myeloma cells. Proc Natl Acad Sci USA. 1998, 95: 162-166. 10.1073/pnas.95.1.162.CrossRef
14.
Henkels KM, Turchi JJ: Cisplatin-induced apoptosis proceeds by caspase-3-dependent and -independent pathways in cisplatin-resistant and -sensitive human ovarian cancer cell lines. Cancer Res. 1999, 59: 3077-3083.
15.
Hamilton G, Cosentini EP, Teleky B, Koperna T, Zacheri J, Riegler M, Feil W, Schiessel R, Wenzi E: The multidrug-resistance modifiers verapamil, cyclosporine A and tamoxifen induce an intracellular acidification in colon carcinoma cell lines in vitro. Anticancer Res. 1993, 13: 2059-2063.
16.
Urbatsch IL, Sankaran B, Weber J, Senior AE: P-glycoprotein is stably inhibited by vanadate-induced trapping of nucleotide at a single catalytic site. J Biol Chem. 1995, 270: 19383-19390. 10.1074/jbc.270.33.19383.CrossRef
17.
Sun YL, Zhou GY, Li KN, Gao P, Zhang QH, Zhen JH, Bai YH, Zhang XF: Suppression of glucosylceramide synthase by RNA interference reverses multidrug resistance in human breast cancer cells. Neoplasma. 2006, 53: 1-8.
18.
Chin KV, Ueda K, Pastan I, Gottesman MM: Modulation of activity of the promoter of the human MDR1 gene by Ras and p53. Science. 1992, 255: 459-462. 10.1126/science.1346476.CrossRef
19.
Nooter K, Boersma AW, Oostrum RG, Burger H, Jochemsen AG, Stoter G: Constitutive expression of the c-H-ras oncogene inhibits doxorubicin-induced apoptosis and promotes cell survival in a rhabdomyosarcoma cell line. Br J Cancer. 1995, 71: 556-561.CrossRef
20.
Di Simone D, Galimberti S, Basolo F, Ciardiello F, Petrini M, Scheper RJ: c-Ha-ras transfection and expression of MDR-related genes in MCF-10A human breast cell line. Anticancer Res. 1997, 17: 3587-3592.
21.
Ejendal KF, Hrycyna CA: Multidrug resistance and cancer: the role of the human ABC transporter ABCG2. Curr Protein Pept Sci. 2002, 3: 503-511. 10.2174/1389203023380521.CrossRef
22.
Eckhardt S: Recent progress in the development of anticancer agents. Curr Med Chem Anticancer Agents. 2002, 2: 419-439. 10.2174/1568011024606389.CrossRef
23.
Choi JH, Lim HY, Joo HJ, Kim HS, Yi JW, Kim HC, Cho YK, Kim MW, Lee KB: Expression of multidrug resistance-associated protein1, P-glycoprotein, and thymidylate synthase in gastric cancer patients treated with 5-fluorouracil and doxorubicin-based adjuvant chemotherapy after curative resection. Br J Cancer. 2002, 86: 1578-1585. 10.1038/sj.bjc.6600305.CrossRef
Metadata
Title
Comparison of the multi-drug resistant human hepatocellular carcinoma cell line Bel-7402/ADM model established by three methods
Authors
Xingguo Zhong
Maoming Xiong
Xiangling Meng
Renhua Gong
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2010
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/1756-9966-29-115

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