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Cancer Chemotherapy and Pharmacology

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01-12-2008 | Original Article

Comparison of the in vitro and in vivo effects of retinoids either alone or in combination with cisplatin and 5-fluorouracil on tumor development and metastasis of melanoma

Authors: Xin Liu, Sui Yung Chan, Paul Chi-Lui Ho

Published in: Cancer Chemotherapy and Pharmacology | Issue 1/2008

Abstract

Purpose

Retinoids have previously been reported to inhibit proliferation of melanoma cell lines in vitro. However, the relative antimetastatic efficacy of various retinoids on melanoma in vivo is unknown. Therefore, we investigated the effects of different retinoids on the invasion and metastasis of murine melanoma B16-F10 cells in vitro and in vivo. Based on the findings, the antitumor effects of a selected retinoid either alone or in combination with cisplatin were also investigated in a preclinical mouse melanoma model.

Methods

Cell proliferation and invasion analyses of murine melanoma B16-F10 cells were assessed in the presence of different retinoids, either alone or in combination with cisplatin (CDDP) or 5-fluorouracil (5-FU). Experimental lung metastasis assay was performed in this study to investigate the antimetastatic efficacy of retinoids. Additionally, a mouse melanoma model was used to assess the antitumor efficacy of a selected retinoid in combination with cisplatin.

Results

Retinoids showed significant antiproliferation and anti-invasion effects on murine melanoma B16-F10 cells. Pretreatment with retinoids increased the sensitivity to CDDP but not to 5-FU in in-vitro. Moreover, the number of metastatic colonies formed in the lungs of mice injected intravenously with B16-F10 cells was significantly reduced by injecting the respective retinoid once a day for 10 days. Treatment with a combination of cisplatin and 13-cis-retinoic acid resulted in a significant reduction in primary tumor size and the number of lung metastatic nodules in melanoma-bearing mice.

Conclusion

These results suggest that retinoids not only exhibit antimetastatic effect, but also enhance the antitumor activity of cisplatin in vivo.

Fidler IJ (1990) Critical factors in the biology of human cancer metastasis: twenty-eighth G.H.A. Clowes memorial award lecture. Cancer Res 50:6130–6138PubMed

Mareel MM, Bracke ME, Storme GA (1985) Mechanisms of tumor spread: a brief overview. In: Grundmann E (ed) The cancer patient: illness and recovery. Gustav Fischer Verlag, Stuttgart, pp 59–64

McKinnell RG (1998) Metastasis. In: McKinnell RG, Parchment RE, Perantoni AO, Pierce GB (eds) The biological basis of cancer. Cambridge University Press, Cambridge, pp 50–78

Stetler Stevenson WG, Liotta LA, Kleiner DE (1994) Extracellular matrix 6: role of matrix metalloproteinases in tumor invasion and metastasis. FASEB J 7:1434–1441

Filder IJ (1978) General considerations for studies of experimental cancer metastasis. Cancer Res 15:399–439

Takenaga M, Igarashi R, Nakayama T, Mizushima Y (1999) Lecithinized ascorbic acid (PC-AS) effectively inhibits murine pulmonary metastasis. Anticancer Res 19:1085–1091PubMed

Leyon PV, Lini CC, Kuttan G (2005) Inhibitory effect of Boerhaavia diffusa on experimental metastasis by B16–F10 melanoma in C57BL/6 mice. Life Sci 76:71339–71349CrossRef

Gava B, Zorzet S, Spessotto P, Cocchietto M, Sava G (2006) Inhibition of B16 melanoma metastases with the ruthenium complex imidazolium trans-imidazoledimethylsulfoxide-tetrachlororuthenate and down-regulation of tumor cell invasion. J Pharmacol Exp Ther 317:284–291PubMedCrossRef

Niles RM (2000) Recent advances in the use of vitamin A (retinoids) in the prevention and treatment of cancer. Nutrition 16:1084–1089PubMedCrossRef

10.

Bertram JS, Vine AL (2005) Cancer prevention by retinoids and carotenoids: independent action on a common target. Biochim Biophys Acta 1740:170–178PubMed

11.

Pasquali D, Rossi V, Bellastella G, Bellastella A, Sinisi AA (2006) Natural and synthetic retinoids in prostate cancer. Curr Pharm Des 12:1923–1929PubMedCrossRef

12.

Siegrist W, Hintermann E, Roggo CN, Apfel CM, Klaus M, Eberle AN (1998) Melanoma cell growth inhibition and melanocortin receptor downregulation induced by selective and non-selective retinoids. Melanoma Res 8:113–122PubMedCrossRef

13.

Ohashi E, Hong SH, Takahashi T, Nakagawa T, Mochizuki M, Nishimura R, Sasak N (2001) Effect of retinoids on growth inhibition of two canine malnoma cell lines. J Vet Med Sci 63:83–86PubMedCrossRef

14.

Emionite L, Galmozzi F, Raffo P, Vergani L, Toma S (2003) Retinoids and malignant melanoma: a pathway of proliferation inhibition on SK MEL28 cell line. Anticancer Res 23:9–13

15.

Wood WR, Seftor EA, Lotan T, Nakajama M, Misiorowski RL, Seftor REB, Lotan R, Hendrix MJC (1990) Retinoic acid inhibits human melanoma tumor cell invasion. Anticancer Res 10:423–432PubMed

16.

Helige C, Smolle J, Zelinig G, Hartmann E, Fink-Puches R, Kerl H, Tritthart HA (1993) Inhibition of K1735–M2 melanoma cell invasion in vitro by retinoic acid. Clin Exp Metastasis 11:409–418PubMedCrossRef

17.

Borden P, Heller RA (1997) Transcriptional control of matrix metalloproteinases and the tissue inhibitors of matrix metalloproteinases. Crit Rev Eukaryot Gene Expr 7:159–178PubMed

18.

Edward M, Gold JA, MacKie RM (1989) Modulation of melanoma cell adhesion to basement membrane components by retinoic acid. J Cell Sci 93:155–161PubMed

19.

Ortiz MA, Bayon Y, Lopez-Hernandez FJ, Piedrafita FJ (2002) Retinoids in combination therapies for the treatment of cancer: mechanisms and perspectives. Drug Resist Update 5:162–175CrossRef

20.

Sacks PG, Harris D, Chou TC (1995) Modulation of growth and proliferation in squamous cell carcinoma by retinoic acid: a rationale for combination therapy with chemotherapeutic agents. Int J Cancer 61:409–415PubMedCrossRef

21.

Recchia F, Lalli A, Lombardo M, De Filippis S, Saggio G, Fabbri F, Rosselli M, Capomolla E, Rea S (2001) Ifosfamide, cisplatin, and 13-cis-retinoic acid for patients with advanced or recurrent squamous cell carcinoma of the head and neck: a phase I-II study. Cancer 92:814–821PubMedCrossRef

22.

Recchia F, De Filippis S, Pompili PL, Rosselli M, Saggio G, Ciorra A, Piccinini M, Rea S (1999) Carboplatin, vindesine, 5-fluorouracil-leucovorin and 13-cis-retinoic acid in the treatment of advanced non-small cell lung cancer. A phase II study. Clin Ther 150:269–274

23.

Shin DM, Glisson BS, Khuri FR, Clifford JL, Clayman G, Benner SE, Forastiere AA, Ginsberg L, Liu D, Lee JJ, Myers J, Goepfert H, Lotan R, Hong WK, Lippman SM (2002) Phase II and biologic study of interferon alfa, retinoic acid, and cisplatin advanced squamous skin cancer. J Clin Oncol 20:364–370PubMedCrossRef

24.

Shin DM, Khuri FR, Murphy B, Garden AS, Clayman G, Francisco M, Liu D, Glisson BS, Glinsberg L, Papadimitrakopoulou V, Myers J, Morrison W, Gillenwater A, Ang KK, Lippman SM, Goepfert H, Hong WK (2001) Combined interferon-α, 13-cis-retinoic acid, and α-tocopherol in locally advanced head and neck squamous cell carcinoma: novel bioadjuvant phase II trial. J Clin Oncol 19:3010–3017PubMed

25.

Csoka K, Tholander B, Gerdin E, de la Torre M, Larsson R, Nygren P (1997) In vitro determination of cytotoxic drug response in ovarian carcinoma using the fluorometric microculture cytotoxicity assay (FMCA). Int J Cancer 72:1008–1012PubMedCrossRef

26.

Melchiori A, Allavena G, Bohm J, Remy W, Schmidt J et al (1987) Interferons inhibit chemotaxis of transformed cells and their invasion of a reconstituted basement membrane. Anticancer Res 7:475–479PubMed

27.

Lotan R (1991) Retinoids as modulators of tumor cell invasion and metastasis. Sem Cancer Biol 2:197–208

28.

Jeten AM (1984) Modulation of cell growth by retinoids and their potential mechanisms of action. Fed Proc 43:134–139

29.

Jacob K, Wach F, Holzapfel U, Hein R, Lengyel E, Buettner R, Bosserhoff AK (1995) In vitro modulation of human melanoma cell invasion and proliferation by all-trans-retinoic acid. Melanoma Res 8:211–219CrossRef

30.

Torgeirsson UP, Lindsay CK, Cottam DW, Gomez DE (1994) Tumor invasion, proteolysis, and angiogenesis. J Neurooncol 18:89–103CrossRef

31.

Mandara M, Nortilli R, Sava T, Cetto GL (2006) Chemotherapy for metastatic melanoma. Expert Rev Anticancer Ther 6:121–130PubMedCrossRef

Title: Comparison of the in vitro and in vivo effects of retinoids either alone or in combination with cisplatin and 5-fluorouracil on tumor development and metastasis of melanoma
Authors: Xin Liu
Sui Yung Chan
Paul Chi-Lui Ho
Publication date: 01-12-2008
Publisher: Springer-Verlag
Published in: Cancer Chemotherapy and Pharmacology / Issue 1/2008
Print ISSN: 0344-5704
Electronic ISSN: 1432-0843
DOI: https://doi.org/10.1007/s00280-008-0763-1

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Abstract

Purpose

Methods

Results

Conclusion

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