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01-04-2020 | Original Article

Comparison of the healing status in suture-less end-to-end intestinal anastomosis using TachoSil with classic two-layer anastomosis in rats

Authors: Hadi Hadavi, Bahram Pourseidi, Shahriar Dabiri, Mohammadreza Ghasemian Moghaddam, Alireza Amirbeigi, Khatereh Dehghani, Amir Hossein Pourdavood, Iman Shamohammadi, Maryam Dehghankhalili, Akram Arabi

Published in: Comparative Clinical Pathology | Issue 2/2020

Abstract

The classical two-layer anastomosis has been experimentally compared with anastomosis using synthetic TachoSil in terms of pre- and post-surgical symptoms and healing status of the anastomotic area in rats. Thirty-six adult male rats were randomly allocated into two groups: in group C (control), classic anastomosis was performed in the ileum of the small intestine, 10 cm away from the ileocecal valve using Vicryl 5-0 interrupted suture; group S (study) received anastomosis in the same area but with TachoSil using three sutures (mesenteric, anti-mesenteric, and anterior wall as suture-less method). The obtained results were analyzed by SPSS20 software, using Chi-square test. Based on the obtained results, group C had more favorable anastomotic healing than group S. Actually, the fibroblast and collagen were seen in most cases of the control rats, while healing was stopped in group S (TachoSil group) in early stages due to thrombosis and vasculitis gangrene. According to the findings, TachoSil patch cannot be advised for safe use in suturing the less small-bowel anastomosis.

Anegg U, Rychlik R, Smolle-Jüttner F (2008) Do the benefits of shorter hospital stay associated with the use of fleece-bound sealing outweigh the cost of the materials? Interact Cardiovasc Thorac Surg 7(2):292–296CrossRef

Babor R, Talbot M, Tyndal A (2009) Treatment of upper gastrointestinal leaks with a removable, covered, self-expanding metallic stent. Surgical Laparoscopy Endoscopy & Percutaneous Techniques 19(1):e1–e4CrossRef

Bhanot S, Alex JC (2002) Current applications of platelet gels in facial plastic surgery. Facial plastic surgery 18(01):027–034CrossRef

Bonanomi G, Prince J, McSteen F, Schauer P, Hamad G (2004) Sealing effect of fibrin glue on the healing of gastrointestinal anastomoses. Surg Endosc Other Interv Tech 18(11):1620–1624

Buchanan GN, Bartram CI, Phillips RK, Gould SW, Halligan S, Rockall TA et al (2003) Efficacy of fibrin sealant in the management of complex anal fistula. Dis Colon Rectum 46(9):1167–1174CrossRef

Cintron JR, Park JJ, Orsay CP, Pearl RK, Nelson RL, Sone JH et al (2000) Repair of fistulas-in-ano using fibrin adhesive. Dis Colon Rectum 43(7):944–949CrossRef

Cueto J, Barrientos T, Rodríguez E, Del Moral P (2011) A new biodegradable adhesive for protection of intestinal anastomoses. Preliminary communication. Arch Med Res 42(6):475–481CrossRef

Erb MA, Claus T, Hartrumpf M, Bachmann S, Albes JM (2009) The use of Tachosil® surgical patch or fibrin glue in coronary artery surgery does not affect quality of anastomosis or provoke postoperative adhesions in pigs. Eur J Cardiothorac Surg 36(4):703–707CrossRef

Horowitz B, Busch M (2008) Estimating the pathogen safety of manufactured human plasma products: application to fibrin sealants and to thrombin. Transfusion. 48(8):1739–1753CrossRef

Jackson MR (2001) Fibrin sealants in surgical practice: an overview. Am J Surg 182(2):S1–S7CrossRef

Karagöz Avcı S, Yüceyar S, Aytac E, Bayraktar O, Erenler I, Ustun H, et al. (2011) Comparison of classical surgery and sutureless repair with DuraSeal or fibrin glue for duodenal perforation in rats

Kaya C, Demir U, Coşkun H, Kalyoncu A, Gündüz B, Eroğlu T et al (2004) Comparison of repair techniques in rat duodonal perforations: simple closure, simple closure and omentoplasty, and fibrin tissue adhesive. Ulusal travma ve acil cerrahi dergisi= Turkish journal of trauma & emergency surgery: TJTES 10(1):11–16

Lau W-Y, Leung K-L, Kwong K-H, Davey IC, Robertson C, Dawson J et al (1996) A randomized study comparing laparoscopic versus open repair of perforated peptic ulcer using suture or sutureless technique. Ann Surg 224(2):131CrossRef

Nordentoft T, Rømer J, Sørensen M (2007) Sealing of gastrointestinal anastomoses with a fibrin glue-coated collagen patch: a safety study. J Investig Surg 20(6):363–369CrossRef

Papavramidis ST, Eleftheriadis EE, Papavramidis TS, Kotzampassi KE, Gamvros OG (2004) Endoscopic management of gastrocutaneous fistula after bariatric surgery by using a fibrin sealant. Gastrointest Endosc 59(2):296–300CrossRef

Papavramidis TS, Kotzampassi K, Kotidis E, Eleftheriadis EE, Papavramidis ST (2008) Endoscopic fibrin sealing of gastrocutaneous fistulas after sleeve gastrectomy and biliopancreatic diversion with duodenal switch. J Gastroenterol Hepatol 23(12):1802–1805CrossRef

Silecchia G, Boru CE, Mouiel J, Rossi M, Anselmino M, Morino M et al (2008) The use of fibrin sealant to prevent major complications following laparoscopic gastric bypass: results of a multicenter, randomized trial. Surg Endosc 22(11):2492–2497CrossRef

Stumpf M, Junge K, Rosch R, Krones C, Klinge U, Schumpelick V (2009) Suture-free small bowel anastomoses using collagen fleece covered with fibrin glue in pigs. J Investig Surg 22(2):138–147CrossRef

Zekavat O, Amanat A, Karami M, Paydar S, Gramizadeh B, Zareian-Jahromi M (2016) Wound healing studies using Punica granatum Peel: an animal experimental study. Adv Skin Wound Care 29(5):217–225CrossRef

Zmora O, Mizrahi N, Rotholtz N, Pikarsky AJ, Weiss EG, Nogueras JJ et al (2003) Fibrin glue sealing in the treatment of perineal fistulas. Dis Colon Rectum 46(5):584–589CrossRef

Title: Comparison of the healing status in suture-less end-to-end intestinal anastomosis using TachoSil with classic two-layer anastomosis in rats
Authors: Hadi Hadavi
Bahram Pourseidi
Shahriar Dabiri
Mohammadreza Ghasemian Moghaddam
Alireza Amirbeigi
Khatereh Dehghani
Amir Hossein Pourdavood
Iman Shamohammadi
Maryam Dehghankhalili
Akram Arabi
Publication date: 01-04-2020
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 2/2020
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-03071-1

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Comparison of the healing status in suture-less end-to-end intestinal anastomosis using TachoSil with classic two-layer anastomosis in rats

Abstract

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Abstract

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