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Archives of Virology
Published in: Archives of Virology 10/2013

01-10-2013 | Brief Report

Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line

Authors: Iveta Matejusova, Katherine Lester, Ziduo Li, Jimena Bravo, Fiona Bland, Bertrand Collet

Published in: Archives of Virology | Issue 10/2013

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Abstract

Salmon pancreas disease virus is an alphavirus (family Togaviridae) affecting mainly Atlantic salmon (Salmo salar L.). Both polyprotein sequences of the Scottish isolate (SAV4640) were determined and compared with those of Irish isolate SAVF93-125. High amino acid sequence similarity (99.4 %) was found. Six amino acid deletions were found in the E2 gene of SAV4640. SAVF93-125 demonstrated a high viral load in culture despite high Mx expression. Approximately 50 % of cells infected with SAVF93-125 exhibited a cytopathic effect by day 8. SAV4640 successfully entered the cells, inducing 10,500-fold higher Mx expression at day 2 compared to SAVF93-25; however, no replication was observed based on results of the nsP1 qRT-PCR.
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Metadata
Title
Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line
Authors
Iveta Matejusova
Katherine Lester
Ziduo Li
Jimena Bravo
Fiona Bland
Bertrand Collet
Publication date
01-10-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 10/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1689-4

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