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01-12-2008 | Original Paper

Comparative Cytokeratin Expression in the Different Cell Types of Salivary Gland Mucoepidermoid Carcinoma

Authors: Rebeca Souza Azevedo, Oslei Paes de Almeida, Luiz Paulo Kowalski, Fábio Ramôa Pires

Published in: Head and Neck Pathology | Issue 4/2008

Abstract

Mucoepidermoid carcinoma is the most common malignant salivary gland tumor, composed of several different cell types, with controversial histogenesis. The aim of this study was to assess the expression of cytokeratins in mucoepidermoid carcinoma, comparing to cytokeratin expression in normal salivary glands, in order to establish a possible correlation between tumor cells immunostaining and mucoepidermoid carcinoma histogenesis and differentiation. Eighty cases of salivary gland mucoepidermoid carcinoma were immunohistochemically examined with the use of antibodies against cytokeratins 6, 7, 8, 13, 14, 18, and 19. Cytokeratin expression varied according to the cellular type: squamous cells presented high expression of cytokeratins 6, 7, 8, 14, 18, and 19; intermediate and mucous cells of cytokeratin 7; clear and columnar cells of cytokeratins 6, 7, 8 and the latter also expressed cytokeratin 18. Cytokeratin 13 expression was low in all cell types. Cytokeratin immunoexpression in mucoepidermoid carcinoma was variable according to the cellular type; but regardless of the cellular type studied, cytokeratins 7 and 13 were, respectively, constantly high and low expressed. The immunoprofile of the normal salivary glands was variable according to the component but, in general, cytokeratin profile in mucoepidermoid carcinoma showed similarity to the immunoexpression on the excretory duct unit of normal salivary glands.

Luna MA. Salivary mucoepidermoid carcinoma: revisited. Adv Anat Pathol. 2006;13:293–307. doi:10.1097/01.pap.0000213058.74509.d3.PubMedCrossRef

Ito FA, Ito K, Vargas PA, et al. Salivary gland tumors in a brazilian population: a retrospective study of 496 cases. Int J Oral Maxillofac Surg. 2005;34:533–6. doi:10.1016/j.ijom.2005.02.005.PubMedCrossRef

Pires FR, Alves FA, de Almeida OP, et al. Carcinoma mucoepidermóide de cabeça e pescoço: estudo clínico-patológico de 173 casos. Rev Bras Otorrinolaringol (Engl Ed). 2002;68:679–84.

Pires FR, de Almeida OP, de Araujo VC, et al. Prognostic factors in head and neck mucoepidermoid carcinoma. Arch Otolaryngol Head Neck Surg. 2004;130:174–80. doi:10.1001/archotol.130.2.174.PubMedCrossRef

Dardick I. Color Atlas/text of salivary gland pathology. New York: Igaku-Shoin Medical Publishers; 1996.

Ellis GL, Auclair PL. Tumors of the salivary glands—Atlas of tumor pathology, 3rd Series, Fascicle 17. Washington: Armed Forces Institute of Pathology; 1996.

Barnes L, Eveson JW, Reichart P, Sidransky D. World health organization classification of tumours. Pathology & genetics—Head and neck tumors. Lyon: IARC Press; 2005.

Dardick I, Daya D, Hardie J, et al. Mucoepidermoid carcinoma: ultrastructural and histogenetic aspects. J Oral Pathol. 1984;13:342–58. doi:10.1111/j.1600-0714.1984.tb01433.x.PubMedCrossRef

Dardick I, Gliniecki MR, Heathcote JG, et al. Comparative histogenesis and morphogenesis of mucoepidermoid carcinoma and pleomorphic adenoma. An ultrastructural study. Virchows Arch A Pathol Anat Histopathol. 1990;417:405–17. doi:10.1007/BF01606029.PubMedCrossRef

10.

Loyola AM, de Sousa SO, Araujo NS, et al. Study of minor salivary gland mucoepidermoid carcinoma differentiation based on immunohistochemical expression of cytokeratins, vimentin and muscle-specific actin. Oral Oncol. 1998;34:112–8. doi:10.1016/S1368-8375(97)00070-5.PubMedCrossRef

11.

Chaudhry AP, Cutler LS, Liefer C, et al. Ultrastructural study of the histogenesis of salivary gland mucoepidermoid carcinoma. J Oral Pathol Med. 1989;18:400–9. doi:10.1111/j.1600-0714.1989.tb01572.x.PubMedCrossRef

12.

Hassanin MB, Ghosh L, Das AK, et al. Immunohistochemical and fluorescent microscopic study of histogenesis of salivary mucoepidermoid carcinoma. J Oral Pathol Med. 1989;18:291–8. doi:10.1111/j.1600-0714.1989.tb00400.x.PubMedCrossRef

13.

Regezi JA, Zarbo RJ, Batsakis JG. Immunoprofile of mucoepidermoid carcinomas of minor salivary glands. Oral Surg Oral Med Oral Pathol. 1991;71:189–92. doi:10.1016/0030-4220(91)90466-P.PubMedCrossRef

14.

da Silveira EJ, Veras Barros SS, de Amorim RF, et al. Cytokeratin profile in mucoepidermoid carcinoma is not related to its histological grading of malignancy. Exp Mol Pathol. 2006;81:72–6. doi:10.1016/j.yexmp.2005.12.001.PubMedCrossRef

15.

Moll R, Franke WW, Schiller DL, et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982;31:11–24. doi:10.1016/0092-8674(82)90400-7.PubMedCrossRef

16.

Coulombe PA, Omary MB. “Hard” and “soft” principles defining the structure, function and regulation of keratin intermediate filaments. Curr Opin Cell Biol. 2002;14:110–22. doi:10.1016/S0955-0674(01)00301-5.PubMedCrossRef

17.

Kirfel J, Magin TM, Reichelt J. Keratins: a structural scaffold with emerging functions. Cell Mol Life Sci. 2003;60:56–71. doi:10.1007/s000180300004.PubMedCrossRef

18.

Sawaf MH, Ouhayoun JP, Forest N. Cytokeratin profiles in oral epithelia: a review and a new classification. J Biol Buccale. 1991;19:187–98.PubMed

19.

Berkovitz BK, Barret AW. Cytokeratin intermediate filaments in oral and odontogenic epithelia. Bull Group Int Rech Sci Stomatol Odontol. 1998;40:4–23.PubMed

20.

Chu PG, Weiss LM. Keratin expression in human tissues and neoplasms. Histopathology. 2002;40:403–39. doi:10.1046/j.1365-2559.2002.01387.x.PubMedCrossRef

21.

de Araujo VC, de Sousa SO, Carvalho YR, et al. Application of immunohistochemistry to the diagnosis of salivary gland tumors. Appl Immunohistochem Mol Morphol. 2000;8:195–202. doi:10.1097/00022744-200009000-00005.PubMedCrossRef

22.

Pires FR, Chen SY, Perez DEC, et al. Cytokeratin expression in central mucoepidermoid carcinoma and glandular odontogenic cyst. Oral Oncol. 2004;40:545–51. doi:10.1016/j.oraloncology.2003.11.007.PubMedCrossRef

23.

Foschini MP, Marucci G, Eusebi V. Low-grade mucoepidermoid carcinoma of salivary glands: characteristic immunohistochemical profile and evidence of striated duct differentiation. Virchows Arch. 2002;440:536–42. doi:10.1007/s00428-001-0585-6.PubMedCrossRef

24.

Sobral AP, Loducca SV, Kowalski LP, et al. Immunohistochemical distinction of high-grade mucoepidermoid carcinoma and epidermoid carcinoma of the parotid region. Oral Oncol. 2002;38:437–40. doi:10.1016/S1368-8375(01)00089-6.PubMedCrossRef

25.

Terada T, Ikeuchi S, Inomoto C, et al. Mucoepidermoid carcinoma of the palate composed exclusively of clear cells (clear cell variant). Virchows Arch. 2004;445:541–3 (Letter to the editor). doi:10.1007/s00428-004-1086-1 PubMedCrossRef

26.

Brannon RB, Willard CC. Oncocytic mucoepidermoid carcinoma of parotid gland origin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:727–33. doi:10.1016/S1079-2104(03)00377-9.PubMedCrossRef

27.

Eversole LR. Histogenic classification of salivary tumors. Arch Pathol. 1971;92:433–43.PubMed

28.

Gustafsson H, Kjorell U, Eriksson A, et al. Distribution of intermediate filament proteins in developing and adult salivary glands in man. Anat Embryol (Berl). 1988;178:243–51. doi:10.1007/BF00318227.CrossRef

29.

Martins MD, Cavalcanti de Araujo V, Raitz R, et al. Expression of cytoskeletal proteins in developing human minor salivary glands. Eur J Oral Sci. 2002;110:316–21. doi:10.1034/j.1600-0722.2002.21360.x.PubMedCrossRef

30.

Meer S, Altini M. CK7+/CK20—immunoexpression profile is typical of salivary gland neoplasia. Histopathology. 2007;51:26–31. doi:10.1111/j.1365-2559.2007.02728.x.PubMedCrossRef

31.

de Araujo VC, de Sousa SO. Expression of different keratins in salivary gland tumours. Eur J Cancer B Oral Oncol. 1996;32B:14–8. doi:10.1016/0964-1955(95)00052-6.PubMed

32.

do Prado RF, Lima CF, Pontes HA, et al. Calcifications in a clear cell mucoepidermoid carcinoma: a case report with histological and immunohistochemical findings. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;104:e-40–4.

33.

Ellis GL. Clear cell neoplasm in salivary glands: clearly a diagnostic challenge. Ann Diagn Pathol. 1998;2:61–78. doi:10.1016/S1092-9134(98)80035-X.PubMedCrossRef

34.

Chen SY. Ultrastructure of mucoepidermoid carcinoma of minor salivary glands. Oral Surg Oral Med Oral Pathol.1979;47:247–55. doi:10.1016/0030-4220(79)90149-X.PubMedCrossRef

Title: Comparative Cytokeratin Expression in the Different Cell Types of Salivary Gland Mucoepidermoid Carcinoma
Authors: Rebeca Souza Azevedo
Oslei Paes de Almeida
Luiz Paulo Kowalski
Fábio Ramôa Pires
Publication date: 01-12-2008
Publisher: Humana Press Inc
Published in: Head and Neck Pathology / Issue 4/2008
Electronic ISSN: 1936-0568
DOI: https://doi.org/10.1007/s12105-008-0074-1

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Comparative Cytokeratin Expression in the Different Cell Types of Salivary Gland Mucoepidermoid Carcinoma

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