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Lasers in Medical Science
Published in: Lasers in Medical Science 1/2017

01-01-2017 | Original Article

Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells

Authors: Jasmin Nurković, Ivan Zaletel, Selmina Nurković, Šefćet Hajrović, Fahrudin Mustafić, Jovan Isma, Aleksandra Jurišić Škevin, Vesna Grbović, Milica Kovačević Filipović, Zana Dolićanin

Published in: Lasers in Medical Science | Issue 1/2017

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Abstract

In recent years, electromagnetic field (EMF) and low-level laser (LLL) have been found to affect various biological processes, the growth and proliferation of cells, and especially that of stem cells. The aim of this study was to investigate the effects of EMF and LLL on proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and thus to examine the impact of these therapeutic physical modalities on stem cell engraftment. hAT-MSCs were isolated from subcutaneous adipose tissue of six persons ranging in age from 21 to 56 years. EMF was applied for a period of 7 days, once a day for 30 min, via a magnetic cushion surface at a frequency of 50 Hz and an intensity of 3 mT. LLL was applied also for 7 days, once a day for 5 min, at radiation energies of 3 J/cm2, with a wavelength of 808 nm, power output of 200 mW, and power density of 0.2 W/cm2. Nonexposed cells (control) were cultivated under the same culture conditions. Seven days after treatment, the cells were examined for cell viability, proliferation, and morphology. We found that after 7 days, the number of EMF-treated hAT-MSCs was significantly higher than the number of the untreated cells, LLL-treated hAT-MSCs were more numerous than EMF-treated cells, and hAT-MSCs that were treated with the combination of EMF and LLL were the most numerous. EMF and/or LLL treatment did not significantly affect hAT-MSC viability by itself. Changes in cell morphology were also observed, in terms of an increase in cell surface area and fractal dimension in hAT-MSCs treated with EMF and the combination of EMF and LLL. In conclusion, EMF and/or LLL treatment accelerated the proliferation of hAT-MSCs without compromising their viability, and therefore, they may be used in stem cell tissue engineering.
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Metadata
Title
Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells
Authors
Jasmin Nurković
Ivan Zaletel
Selmina Nurković
Šefćet Hajrović
Fahrudin Mustafić
Jovan Isma
Aleksandra Jurišić Škevin
Vesna Grbović
Milica Kovačević Filipović
Zana Dolićanin
Publication date
01-01-2017
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 1/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-2097-2

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