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Cancer Cell International

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Open Access 01-12-2021 | Colorectal Cancer | Primary research

XPF expression and its relationship with the risk and prognosis of colorectal cancer

Authors: Huixin Hu, Jingjing Jing, Xiaodong Lu, Yuan Yuan, Chengzhong Xing

Published in: Cancer Cell International | Issue 1/2021

Abstract

Background

XPF (xeroderma pigmentosum complementation group F) is a key factor contributing to DNA damage excision of nucleotide excision repair pathway. The relationship between XPF expression and the risk and prognosis of colorectal cancer (CRC) is unclear.

Methods

In this experiment, a total of 824 cases of colorectal tissue were collected. XPF protein expression was detected by immunohistochemical staining. We conducted a Mann–Whitney U test in order to explore the differential expression of XPF between CRC and non-cancer controls, and the correlation between XPF expression and CRC clinicopathological parameters. Univariate and multivariate Cox regression analyses were conducted to investigate the relationship between XPF expression and CRC prognosis. The Java based software GSEA as well as STRING, David, GO, KEGG were used to explore the function and regulation network of XPF.

Results

The results demonstrated that the XPF expression in CRC was significantly up-regulated compared with non-tumor controls (P < 0.001) and adenoma tissue (P < 0.001). XPF protein was increased in the dynamic sequence of anal diseases to adenoma tissue to CRC. Expression of XPF was related to tumor location (P = 0.005) and tumor growth pattern (P = 0.009). The results of prognosis analysis suggested that in patients with stage T1-T2, XPF low expression may be significantly associated with better overall survival (HR = 7.978, 95% CI 1.208–52.673, P = 0.031). XPF and its interacting genes played a vital role in different processes of nucleotide excision repair pathway. XPF expression was related with Ubiquitin like protein specific protease activity.

Conclusions

XPF might be a promising biomarker for CRC risk, and also showed potential as a prognostic predictor in CRC patients.

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Title: XPF expression and its relationship with the risk and prognosis of colorectal cancer
Authors: Huixin Hu
Jingjing Jing
Xiaodong Lu
Yuan Yuan
Chengzhong Xing
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Colorectal Cancer
Colorectal Cancer
Published in: Cancer Cell International / Issue 1/2021
Electronic ISSN: 1475-2867
DOI: https://doi.org/10.1186/s12935-020-01710-0

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Abstract

Background

Methods

Results

Conclusions

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