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Cancer Cell International
Published in: Cancer Cell International 1/2021

Open Access 01-12-2021 | Colorectal Cancer | Primary research

Protein–protein interaction analysis reveals a novel cancer stem cell related target TMEM17 in colorectal cancer

Authors: Zhao-liang Yu, Yu-feng Chen, Bin Zheng, Ze-rong Cai, Yi-feng Zou, Jia Ke, Ping Lan, Feng Gao, Xiao-jian Wu

Published in: Cancer Cell International | Issue 1/2021

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Abstract

Background

Cancer stem cells (CSCs) are a small subpopulation of cells within tumors with stem cell property. Increased evidence suggest that CSCs could be responsible for chemoresistance and recurrence in colorectal cancer (CRC). However, a reliable therapeutic target on CSCs is still lacking.

Methods

Here we describe a two-step strategy to generate CSC targets with high selectivity for colon stem cell markers, specific proteins that are interacted with CSC markers were selected and subsequently validated in a survival analysis. TMEM17 protein was found and its biological functions in CRC cells were further examined. Finally, we utilized the Gene Set Enrichment Analysis (GSEA) to investigate the potential mechanisms of TMEM17 in CRC.

Results

By combining protein–protein interaction (PPI) database and high-throughput gene profiles, network analysis revealed a cluster of colon CSCs related genes. In the cluster, TMEM17 was identified as a novel CSCs related gene. The results of in-vitro functional study demonstrated that TMEM17 depletion can suppress the proliferation of CRC cells and sensitize CRC cells to chemotherapy drugs. Enrichment analysis revealed that the expression of TMEM17 is associated with the magnitude of activation of the Wnt/β-catenin pathway. Further validation in clinical samples demonstrated that the TMEM17 expression was much higher in tumor than normal tissue and was associated with poor survival in CRC patients.

Conclusion

Collectively, our finding unveils the critical role of TMEM17 in CRC and TMEM17 could be a potential effective therapeutic target for tumor recurrence and chemoresistance in the colorectal cancer (CRC).
Appendix
Available only for authorised users
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Metadata
Title
Protein–protein interaction analysis reveals a novel cancer stem cell related target TMEM17 in colorectal cancer
Authors
Zhao-liang Yu
Yu-feng Chen
Bin Zheng
Ze-rong Cai
Yi-feng Zou
Jia Ke
Ping Lan
Feng Gao
Xiao-jian Wu
Publication date
01-12-2021
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2021
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-021-01794-2

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