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Cancer Cell International
Published in: Cancer Cell International 1/2021

Open Access 01-12-2021 | Colorectal Cancer | Primary research

Anticancer effects of bifidobacteria on colon cancer cell lines

Authors: Zeinab Faghfoori, Mohammad Hasan Faghfoori, Amir Saber, Azimeh Izadi, Ahmad Yari Khosroushahi

Published in: Cancer Cell International | Issue 1/2021

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Abstract

Background

Colorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and has uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using it as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines.

Methods

The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method.

Results

The cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes.

Conclusions

In the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.
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Metadata
Title
Anticancer effects of bifidobacteria on colon cancer cell lines
Authors
Zeinab Faghfoori
Mohammad Hasan Faghfoori
Amir Saber
Azimeh Izadi
Ahmad Yari Khosroushahi
Publication date
01-12-2021
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2021
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-021-01971-3

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