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BMC Complementary Medicine and Therapies

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Open Access 01-12-2020 | Colon Cancer | Research article

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Authors: Rawan Al-Nemari, Abdulrahman Al-Senaidy, Abdelhabib Semlali, Mohammad Ismael, Ahmed Yacine Badjah-Hadj-Ahmed, Abir Ben Bacha

Published in: BMC Complementary Medicine and Therapies | Issue 1/2020

Abstract

Background

The research and application of plants in food supplements and drugs have attracted great interest. This study aimed to examine the efficiency of several solvents for the extraction of the main compounds from Annona squamosa leaves and to evaluate the antioxidant, antibacterial, and anticancer activities of these extracts.

Methods

Gas chromatography-mass spectrometry was used to screen the bioactive compounds of A. squamosa methanolic extract. The free radical, hydrogen peroxide, and nitric oxide scavenging activities of the extracts were investigated. Furthermore, MTT, nuclear staining, LDH, and monolayer wound repair assays were performed to evaluate the potential anticancer activity of the extracts in colon cancer cells while the antibacterial activity was tested by using a well diffusion assay.

Results

A. squamosa leaves extracts were found to contain several bioactive compounds, of which the majority were sesquiterpenes (C₁₅H₂₄). These extracts exhibited strong antioxidant activity and antibacterial potency against both gram-positive and gram-negative bacteria. Different A. squamosa leaves extracts displayed remarkable antiproliferative, cytotoxic, antimigration, and apoptotic activities in colon cancer cells.

Conclusions

A. squamosa leaves contain major bioactive compounds that inhibit the growth of several types of bacteria and colon cancer cell lines, which demonstrated their efficacy as an alternative source of antibiotics and for the development of novel drugs for colon cancer therapy.

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Title: GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves
Authors: Rawan Al-Nemari
Abdulrahman Al-Senaidy
Abdelhabib Semlali
Mohammad Ismael
Ahmed Yacine Badjah-Hadj-Ahmed
Abir Ben Bacha
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Colon Cancer
Colon Cancer
Published in: BMC Complementary Medicine and Therapies / Issue 1/2020
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-020-03029-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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