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Open Access 01-12-2013 | Research

Clinical significance of combined detection of human papilloma virus infection and human telomerase RNA component gene amplification in patients with squamous cell carcinoma of the esophagus in northern China

Authors: Yu-Feng Wang, Xin-Shuai Wang, She-Gan Gao, Qiang Chen, Yan-Tong Yang, Zhong-Yue Xiao, Xiu-Qing Peng, Xiu-Feng Hu, Qia-Yin Wang, Xiao-Shan Feng

Published in: European Journal of Medical Research | Issue 1/2013

Abstract

Background

The aim of the study was to test for human papilloma virus (HPV) infection and human telomerase RNA component (hTERC) gene amplification in tissues derived from esophageal cancer, in esophagus displaying atypical hyperplasia and in normal tissue, and to analyze the relationship between them and discuss whether HPV infection and hTERC gene amplification play a role in the duration of survival of esophageal cancer patients.

Methods

To test for HPV infection, surface plasma resonance was used after extracting and subjecting the DNA to PCR amplification. Measurement of hTERC gene amplification was performed by the fluorescence in situ hybridization technique.

Results

The rates of HPV infection in the normal group, the atypical esophageal hyperplasia group and the cancer group were 0% (0/40), 10.00% (1/10) and 20.65% (19/92), respectively, with a statistically significant difference of P < 0.01. The hTERC gene amplification rate in normal tissue, grade I atypical hyperplastic tissue, grade II/III atypical hyperplastic tissue and esophageal cancer tissue were 0% (0/89), 15.38% (4/26), 47.06% (8/17) and 89.13% (82/92), respectively, with a statistically significant difference of P < 0.01. On follow-up of 92 patients, survival curves of the HPV-positive and HPV-negative groups were not significantly different (P > 0.05). Survival curves of the hTERC gene amplification-positive and hTERC gene amplification-negative groups were statistically significant (P < 0.05). A matching chi-square test showed that there was no correlation between HPV infection and hTERC gene amplification (P > 0.05).

Conclusion

HPV infection may be one of many factors contributing to the development of esophageal cancer, but it does not influence prognosis. Amplification of the hTERC gene appears to influence certain features associated with postoperative survival in esophageal carcinoma patients.

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Title: Clinical significance of combined detection of human papilloma virus infection and human telomerase RNA component gene amplification in patients with squamous cell carcinoma of the esophagus in northern China
Authors: Yu-Feng Wang
Xin-Shuai Wang
She-Gan Gao
Qiang Chen
Yan-Tong Yang
Zhong-Yue Xiao
Xiu-Qing Peng
Xiu-Feng Hu
Qia-Yin Wang
Xiao-Shan Feng
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2013
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/2047-783X-18-11

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Clinical significance of combined detection of human papilloma virus infection and human telomerase RNA component gene amplification in patients with squamous cell carcinoma of the esophagus in northern China

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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