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01-03-2022 | Cleft Lip and Palate | Original Article

Candida species biotypes and polyclonality of potentially virulent Candida albicans isolated from oral cavity of patients with orofacial clefts

Authors: Marcelo Fabiano Gomes Boriollo, Mateus Cardoso Oliveira, Vanessa Bassinello, Paula Cristina Aníbal, Thaísla Andrielle da Silva, Jeferson Júnior da Silva, Rodrigo Carlos Bassi, Manoel Francisco Rodrigues Netto, Carlos Tadeu dos Santos Dias, José Francisco Höfling

Published in: Clinical Oral Investigations | Issue 3/2022

Abstract

Objectives

This study evaluated the incidence of Candida species, and the genetic diversity and virulence of C. albicans of the oral cavity from patients with cleft lip and palate (CLP).

Materials and methods

Oral samples were investigated by microbiological and species-specific PCR methods. The genetic diversity of C. albicans was established using isoenzyme markers, Nei’s statistics, and clustering analysis. Hydrolytic enzymes (SAPs and PLs) were analyzed in vitro.

Results

Oral colonization by Candida species was observed in 29 patients with CLP (65.9%), and C. albicans was highly prevalent. SAP and PL activities were observed in 100% and 51.9% of isolates, respectively. High genetic diversity and patterns of monoclonal and polyclonal oral colonization by C. albicans were observed among patients with CLP. Two major polymorphic taxa (A and B) and other minor polymorphic taxa (C to J) were identified. Only one of the 16 clusters (taxon A) harbored strains from patients with and without CLP, whereas other clusters harbored strains exclusively from CLP patients.

Conclusions

The anatomical conditions of the oral cavity of patients with CLP contribute to the high incidence of Candida species (C. albicans, C. krusei, C. tropicalis, and/or Candida spp.). Data suggest high genetic diversity of potentially virulent C. albicans strains in the oral cavity of CLP patients.

Clinical relevance

Microbiological niches in orofacial clefts can contribute to the emergence of a relative clinical genotypic identity of C. albicans. However, orofacial rehabilitation centers can contribute to the direct and indirect sources of transmission and propagation of Candida species.

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Title: Candida species biotypes and polyclonality of potentially virulent Candida albicans isolated from oral cavity of patients with orofacial clefts
Authors: Marcelo Fabiano Gomes Boriollo
Mateus Cardoso Oliveira
Vanessa Bassinello
Paula Cristina Aníbal
Thaísla Andrielle da Silva
Jeferson Júnior da Silva
Rodrigo Carlos Bassi
Manoel Francisco Rodrigues Netto
Carlos Tadeu dos Santos Dias
José Francisco Höfling
Publication date: 01-03-2022
Publisher: Springer Berlin Heidelberg
Keywords: Cleft Lip and Palate
Cleft Lip and Palate
Cleft Lip and Palate
Cleft Lip and Palate
Cleft Lip and Palate
Published in: Clinical Oral Investigations / Issue 3/2022
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-021-04290-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Candida species biotypes and polyclonality of potentially virulent Candida albicans isolated from oral cavity of patients with orofacial clefts

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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