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General Thoracic and Cardiovascular Surgery
Published in: General Thoracic and Cardiovascular Surgery 7/2023

09-02-2023 | Original Article

Circ_0024108 promotes the progression of esophageal cancer cells

Authors: Tongchen Hu, Huali Peng, Fan Yang, Fan Zhang, Jintao He

Published in: General Thoracic and Cardiovascular Surgery | Issue 7/2023

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Abstract

Background

Esophageal squamous cell carcinoma (ESCC) is a serious malignant cancer. The treatment effect of ESCC is relatively poor and needs further improvement. According to reports, circular RNAs (circRNAs) actively participate in human carcinogenesis. More explorations are needed about the action of circRNAs in ESCC.

Methods

Circ_0024108, miR-488-3p, and USP14 was quantified by a qRT-PCR or immunoblotting method. Cell proliferation evaluation was performed by MTT, EdU, and colony formation assays. Evaluation of cell motility and invasiveness was conducted using wound healing assay and transwell assay. The regulatory mechanism of circ_0024108, miR-488-3p, and USP14 was detected by RNA pull-down assay and dual-luciferase reporter assay.

Results

Circ_0024108 and USP14 were significantly overexpressed in ESCC, while miR-488-3p was underexpressed. Deficiency of circ_0024108 impeded cell growth, motility, and invasiveness. Circ_0024108 regulated the expression of USP14 in ESCC cells via miR-488-3p. Also, circ_0024108 was present at high levels in serum exosomes from ESCC patients with high specificity and sensitivity.

Conclusions

Taken together, circ_0024108 participated in the progress of ESCC through the miR-488-3p/USP14 axis. Circ_0024108 was differentially expressed in serum exosomes. Circ_0024108 might be a potential biomarker for the diagnosis of ESCC.
Appendix
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Metadata
Title
Circ_0024108 promotes the progression of esophageal cancer cells
Authors
Tongchen Hu
Huali Peng
Fan Yang
Fan Zhang
Jintao He
Publication date
09-02-2023
Publisher
Springer Nature Singapore
Published in
General Thoracic and Cardiovascular Surgery / Issue 7/2023
Print ISSN: 1863-6705
Electronic ISSN: 1863-6713
DOI
https://doi.org/10.1007/s11748-023-01909-8

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