Published in:
Open Access
01-12-2017 | Research article
Chemical composition and evaluation of the antibacterial and Cytotoxic activities of the essential oil from the leaves of Myracrodruon urundeuva
Authors:
Ítalo Diego Rebouças de Araújo, Nayara Coriolano de Aquino, Andreza Conceição Véras de Aguiar Guerra, Renato Ferreira de Almeida Júnior, Renata Mendonça Araújo, Raimundo Fernandes de Araújo Júnior, Kléber Juvenal Silva Farias, José Veríssimo Fernandes, Vânia Sousa Andrade
Published in:
BMC Complementary Medicine and Therapies
|
Issue 1/2017
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Abstract
Background
This study evaluated the in vitro activity of essential oil extracted from the leaves of Myracrodruon urundeuva.
Methods
The oil was obtained by hydro-distillation and characterized by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detector (GC-FID). The antibacterial activity was evaluated by the broth microdilution technique and the MIF was determined by using growth indicator CTT (2,3,5-triphenyl-tetrazolium) and CBM in BHI agar. The oil’s cytotoxicity was evaluated in HeLa, HEK-293, and Vero E6 cells using MTT, 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium.
Results
The oil showed chemical markers, including α-pinene (87.85%), trans-caryophyllene (1.57%), limonene (1.49%) and β -pinene (1.42%), and activity against all strains: Staphylococcus aureus (MIC = MBC = 0.22 mg/mL), Staphylococcus epidermidis (MIC = 0.11 mg/mL and MBC = 0.22 mg/mL), Escherichia coli (MIC = 0.88 mg/mL and MBC = 1.75 mg/mL), Pseudomonas aeruginosa (MIC = MBC = 7 mg/mL) and Salmonella Enteritidis (MIC = MBC = 0.44 mg/mL). In vitro cytotoxicity tests showed that the oil is not toxic and has slight antitumor activity.
Conclusions
We conclude that the M. urundeuva oil results are promising, with prospects of being pharmacologically viable.